cDNA Clone CRTC1

• Synonyms: TORC1
• Plasmid: pGEX6P-1-CRTC1
• Parent Plasmid: pGEX6P1
• Expressed: GST-CRTC1
• Genbank: AY360171
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGACTTCGAACAATCCGCGGAAATTCAGCGAGAAGATCGC
GCTGCACAATCAGAAGCAGGCGGAGGAGACGGCGGCCTTCGAGGAGGTCA
TGAAGGACCTGAGCCTGACGCGGGCCGCGCGGCTCCAGCTCCAGAAATCC
CAGTACCTGCAACTGGGCCCCAGCCGAGGCCAGTACTATGGCGGGTCCCT
GCCCAACGTGAACCAGATCGGGAGTGGCACCATGGACCTGCCCTTCCAGA
CCCCCTTCCAATCCTCGGGCCTGGACACCAGCCGGACCACCCGGCACCAT
GGGCTGGTGGACAGGGTGTACCGGGAGCGTGGCCGGCTCGGCTCCCCACA
CCGCCGGCCCCTGTCAGTGGACAAACACGGACGGCAGGCCGACAGCTGCC
CCTATGGCACCATGTACCTCTCACCACCCGCGGACACCAGCTGGAGAAGG
ACCAATTCTGACTCCGCCCTGCACCAGAGCACAATGACGCCCACGCAGCC
AGAATCCTTTAGCAGTGGGTCCCAGGACGTGCACCAGAAAAGAGTCTTAC
TGTTAACAGTCCCAGGAATGGAAGAGACCACATCAGAGGCAGACAAAAAC
CTTTCCAAGCAAGCATGGGACACCAAGAAGACGGGGTCCAGGCCCAAGTC
CTGTGAGGTCCCCGGAATCAACATCTTCCCGTCTGCCGACCAGGAAAACA
CTACAGCCCTGATCCCCGCCACCCACAACACAGGGGGGTCCCTGCCCGAC
CTGACCAACATCCACTTCCCCTCCCCGCTCCCGACCCCGCTGGACCCCGA
GGAGCCCACCTTCCCTGCACTGAGCAGCTCCAGCAGCACCGGCAACCTCG
CGGCCAACCTGACGCACCTGGGCATCGGTGGCGCCGGCCAGGGAATGAGC
ACACCTGGCTCCTCTCCACAGCACCGCCCAGCTGGCGTCAGCCCCCTGTC
CCTGAGCACAGAGGCAAGGCGTCAGCAGGCATCGCCCACCCTGTCCCCGC
TGTCACCCATCACTCAGGCTGTAGCCATGGACGCCCTGTCTCTGGAGCAG
CAGCTGCCCTACGCCTTCTTCACCCAGGCGGGCTCCCAGCAGCCACCGCC
GCAGCCCCAGCCCCCGCCGCCTCCTCCACCCGCGTCCCAGCAGCCACCAC
CCCCGCCACCCCCACAGGCGCCCGTCCGCCTGCCCCCTGGTGGCCCCCTG
TTGCCCAGCGCCAGCCTGACTCGTGGGCCACAGCCGCCCCCGCTTGCAGT
CACGGTACCGTCCTCTCTCCCCCAGTCCCCCCCAGAGAACCCTGGCCAGC
CATCGATGGGGATCGACATCGCCTCGGCGCCGGCTCTGCAGCAGTACCGC
ACTAGCGCCGGCTCCCCGGCCAACCAGTCTCCCACCTCGCCAGTCTCCAA
TCAAGGCTTCTCCCCAGGGAGCTCCCCGCAACACACTTCCACCCTGGGCA
GCGTGTTTGGGGACGCGTACTATGAGCAGCAGATGGCGGCCAGGCAGGCC
AATGCTCTGTCCCACCAGCTGGAGCAGTTCAACATGATGGAGAACGCCAT
CAGCTCCAGCAGCCTGTACAGCCCGGGCTCCACACTCAACTACTCGCAGG
CGGCCATGATGGGCCTCACGGGCAGCCACGGGAGCCTGCCGGACTCGCAG
CAACTGGGATACGCCAGCCACAGTGGCATCCCCAACATCATCCTCACAGT
GACAGGAGAGTCCCCCCCCAGCCTCTCTAAAGAACTGACCAGCTCTCTGG
CCGGGGTCGGCGACGTCAGCTTCGACTCCGACAGCCAGTTTCCCCTGGAC
GAACTCAAGATCGACCCCCTGACCCTCGACGGACTGCACATGCTCAACGA
CCCCGACATGGTTCTGGCCGACCCAGCCACCGAGGACACCTTCCGGATGG
ACCGCCTGTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATSNNPRKFSEKIALHNQ
KQAEETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVN
QIGSGTMDLPFQTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPL
SVDKHGRQADSCPYGTMYLSPPADTSWRRTNSDSALHQSTMTPTQPESFS
SGSQDVHQKRVLLLTVPGMEETTSEADKNLSKQAWDTKKTGSRPKSCEVP
GINIFPSADQENTTALIPATHNTGGSLPDLTNIHFPSPLPTPLDPEEPTF
PALSSSSSTGNLAANLTHLGIGGAGQGMSTPGSSPQHRPAGVSPLSLSTE
ARRQQASPTLSPLSPITQAVAMDALSLEQQLPYAFFTQAGSQQPPPQPQP
PPPPPPASQQPPPPPPPQAPVRLPPGGPLLPSASLTRGPQPPPLAVTVPS
SLPQSPPENPGQPSMGIDIASAPALQQYRTSAGSPANQSPTSPVSNQGFS
PGSSPQHTSTLGSVFGDAYYEQQMAARQANALSHQLEQFNMMENAISSSS
LYSPGSTLNYSQAAMMGLTGSHGSLPDSQQLGYASHSGIPNIILTVTGES
PPSLSKELTSSLAGVGDVSFDSDSQFPLDELKIDPLTLDGLHMLNDPDMV
LADPATEDTFRMDRL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterail expression. As genbank except for Δ82-98. This is believed to be the correct sequence.