cDNA Clone CRTC1

• Synonyms: TORC1
• Plasmid: pFBHTb CRTC1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-CRTC1
• Genbank: AY360171
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGACTTCGAACAATCCGCGGAAATTCAGCGAGAAGATCGC
GCTGCACAATCAGAAGCAGGCGGAGGAGACGGCGGCCTTCGAGGAGGTCA
TGAAGGACCTGAGCCTGACGCGGGCCGCGCGGCTCCAGCTCCAGAAATCC
CAGTACCTGCAACTGGGCCCCAGCCGAGGCCAGTACTATGGCGGGTCCCT
GCCCAACGTGAACCAGATCGGGAGTGGCACCATGGACCTGCCCTTCCAGA
CCCCCTTCCAATCCTCGGGCCTGGACACCAGCCGGACCACCCGGCACCAT
GGGCTGGTGGACAGGGTGTACCGGGAGCGTGGCCGGCTCGGCTCCCCACA
CCGCCGGCCCCTGTCAGTGGACAAACACGGACGGCAGGCCGACAGCTGCC
CCTATGGCACCATGTACCTCTCACCACCCGCGGACACCAGCTGGAGAAGG
ACCAATTCTGACTCCGCCCTGCACCAGAGCACAATGACGCCCACGCAGCC
AGAATCCTTTAGCAGTGGGTCCCAGGACGTGCACCAGAAAAGAGTCTTAC
TGTTAACAGTCCCAGGAATGGAAGAGACCACATCAGAGGCAGACAAAAAC
CTTTCCAAGCAAGCATGGGACACCAAGAAGACGGGGTCCAGGCCCAAGTC
CTGTGAGGTCCCCGGAATCAACATCTTCCCGTCTGCCGACCAGGAAAACA
CTACAGCCCTGATCCCCGCCACCCACAACACAGGGGGGTCCCTGCCCGAC
CTGACCAACATCCACTTCCCCTCCCCGCTCCCGACCCCGCTGGACCCCGA
GGAGCCCACCTTCCCTGCACTGAGCAGCTCCAGCAGCACCGGCAACCTCG
CGGCCAACCTGACGCACCTGGGCATCGGTGGCGCCGGCCAGGGAATGAGC
ACACCTGGCTCCTCTCCACAGCACCGCCCAGCTGGCGTCAGCCCCCTGTC
CCTGAGCACAGAGGCAAGGCGTCAGCAGGCATCGCCCACCCTGTCCCCGC
TGTCACCCATCACTCAGGCTGTAGCCATGGACGCCCTGTCTCTGGAGCAG
CAGCTGCCCTACGCCTTCTTCACCCAGGCGGGCTCCCAGCAGCCACCGCC
GCAGCCCCAGCCCCCGCCGCCTCCTCCACCCGCGTCCCAGCAGCCACCAC
CCCCGCCACCCCCACAGGCGCCCGTCCGCCTGCCCCCTGGTGGCCCCCTG
TTGCCCAGCGCCAGCCTGACTCGTGGGCCACAGCCGCCCCCGCTTGCAGT
CACGGTACCGTCCTCTCTCCCCCAGTCCCCCCCAGAGAACCCTGGCCAGC
CATCGATGGGGATCGACATCGCCTCGGCGCCGGCTCTGCAGCAGTACCGC
ACTAGCGCCGGCTCCCCGGCCAACCAGTCTCCCACCTCGCCAGTCTCCAA
TCAAGGCTTCTCCCCAGGGAGCTCCCCGCAACACACTTCCACCCTGGGCA
GCGTGTTTGGGGACGCGTACTATGAGCAGCAGATGGCGGCCAGGCAGGCC
AATGCTCTGTCCCACCAGCTGGAGCAGTTCAACATGATGGAGAACGCCAT
CAGCTCCAGCAGCCTGTACAGCCCGGGCTCCACACTCAACTACTCGCAGG
CGGCCATGATGGGCCTCACGGGCAGCCACGGGAGCCTGCCGGACTCGCAG
CAACTGGGATACGCCAGCCACAGTGGCATCCCCAACATCATCCTCACAGT
GACAGGAGAGTCCCCCCCCAGCCTCTCTAAAGAACTGACCAGCTCTCTGG
CCGGGGTCGGCGACGTCAGCTTCGACTCCGACAGCCAGTTTCCCCTGGAC
GAACTCAAGATCGACCCCCTGACCCTCGACGGACTGCACATGCTCAACGA
CCCCGACATGGTTCTGGCCGACCCAGCCACCGAGGACACCTTCCGGATGG
ACCGCCTGTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMATSNNPRKFSEKIALHNQKQA
EETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVNQIG
SGTMDLPFQTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPLSVD
KHGRQADSCPYGTMYLSPPADTSWRRTNSDSALHQSTMTPTQPESFSSGS
QDVHQKRVLLLTVPGMEETTSEADKNLSKQAWDTKKTGSRPKSCEVPGIN
IFPSADQENTTALIPATHNTGGSLPDLTNIHFPSPLPTPLDPEEPTFPAL
SSSSSTGNLAANLTHLGIGGAGQGMSTPGSSPQHRPAGVSPLSLSTEARR
QQASPTLSPLSPITQAVAMDALSLEQQLPYAFFTQAGSQQPPPQPQPPPP
PPPASQQPPPPPPPQAPVRLPPGGPLLPSASLTRGPQPPPLAVTVPSSLP
QSPPENPGQPSMGIDIASAPALQQYRTSAGSPANQSPTSPVSNQGFSPGS
SPQHTSTLGSVFGDAYYEQQMAARQANALSHQLEQFNMMENAISSSSLYS
PGSTLNYSQAAMMGLTGSHGSLPDSQQLGYASHSGIPNIILTVTGESPPS
LSKELTSSLAGVGDVSFDSDSQFPLDELKIDPLTLDGLHMLNDPDMVLAD
PATEDTFRMDRL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3541
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression. As genbank except for Δ82-98. This is believed to be the correct sequence.