cDNA Clone GRB14

• Plasmid: pEBG6P-GRB14
• Parent Plasmid: pEBG6P
• Expressed: GST-GRB14
• Genbank: NM_004490
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACTAGTACCACTTCCCTGCAAGATGGGCAGAGCGCCGCGAGCAG
GGCGGCTGCCCGGGATTCGCCGCTGGCCGCCCAGGTGTGTGGCGCTGCCC
AGGGGAGGGGCGACGCCCACGACCTGGCGCCGGCCCCCTGGCTGCACGCG
CGAGCGCTCCTGCCCCTTCCGGACGGGACCCGCGGCTGTGCTGCAGACAG
GAGAAAAAAGAAAGATCTTGATGTTCCGGAAATGCCATCTATTCCAAACC
CTTTTCCTGAGCTATGCTGTTCTCCAATTACATCTGTGTTGTCAGCAGAC
CTATTTCCCAAAGCAAATTCAAGGAAAAAACAGGTGATTAAAGTATACAG
TGAAGATGAAACCAGCAGGGCTTTAGATGTACCCAGTGACATAACGGCTC
GAGATGTTTGTCAGCTGTTGATCCTGAAGAATCATTACATTGATGACCAC
AGCTGGACCCTTTTTGAGCACCTGCCTCACATAGGTGTAGAAAGAACAAT
AGAAGACCACGAACTGGTGATTGAAGTGCTATCCAACTGGGGGATAGAAG
AAGAAAACAAACTATACTTTAGAAAAAATTATGCCAAATATGAGTTCTTT
AAAAACCCAATGTATTTTTTTCCAGAGCATATGGTATCTTTTGCAACTGA
AACCAATGGTGAAATATCCCCCACACAGATTTTGCAGATGTTTCTGAGTT
CAAGCACATATCCTGAAATTCATGGTTTCTTACATGCGAAAGAACAGGGA
AAGAAGTCTTGGAAAAAAATTTACTTTTTTCTAAGAAGATCTGGTTTATA
TTTTTCTACTAAAGGAACATCAAAGGAACCGCGGCATTTGCAGTTTTTCA
GCGAATTTGGCAATAGTGATATTTATGTGTCACTGGCAGGCAAAAAAAAA
CATGGAGCACCGACTAACTATGGATTCTGCTTTAAGCCTAACAAAGCGGG
AGGGCCCCGAGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGTAGGA
CGTGCTGGGTGACCGCGATTAGATTGCTTAAGTATGGCATGCAGCTGTAC
CAGAATTATATGCATCCATATCAAGGTAGAAGTGGCTGCAGTTCACAGAG
CATATCACCTATGAGAAGTATATCAGAGAATTCCCTGGTAGCAATGGACT
TCTCAGGCCAGAAAAGCAGAGTTATAGAAAATCCCACTGAAGCCCTTTCA
GTTGCGGTTGAAGAAGGACTCGCTTGGAGGAAAAAAGGATGTTTACGCCT
GGGCACTCACGGTAGCCCCACTGCCTCTTCACAGAGCTCTGCCACAAACA
TGGCTATCCACCGGTCCCAGCCATGGTTTCACCACAAAATTTCTAGAGAT
GAGGCTCAGCGATTGATTATTCAGCAAGGACTTGTGGATGGAGTTTTCTT
GGTACGGGATAGTCAGAGTAACCCCAAAACTTTCGTACTGTCAATGAGTC
ATGGACAAAAAATAAAGCACTTTCAAATTATACCAGTAGAAGATGACGGT
GAAATGTTCCACACACTGGATGATGGCCACACAAGATTTACAGATCTAAT
ACAGCTGGTGGAGTTCTATCAACTCAATAAGGGCGTTCTTCCTTGCAAGT
TGAAACATTATTGTGCTAGGATTGCTCTCTAGGGTACC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSTTSLQDGQSAA
SRAAARDSPLAAQVCGAAQGRGDAHDLAPAPWLHARALLPLPDGTRGCAA
DRRKKKDLDVPEMPSIPNPFPELCCSPITSVLSADLFPKANSRKKQVIKV
YSEDETSRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGVER
TIEDHELVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFA
TETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSG
LYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNK
AGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSS
QSISPMRSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCL
RLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGV
FLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTD
LIQLVEFYQLNKGVLPCKLKHYCARIAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3526
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.