cDNA Clone ROCK2 (rat)

• Synonyms: ROCK2
• Plasmid: pFB GST ROCK2 D544-1380
• Parent Plasmid: pFastbac GST
• Expressed: GST-ROCK2 D544-1380
• Genbank: NM_013022
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCCCCGGCGCCCCCGAGGCCGCGCCGAGGGACGGGGCGGGCGCGGG
TCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCA
ACGTGGAAAGCCTGCTGGATGGCTTAAATTCCTTGGTCCTTGATTTGGAT
TTTCCTGCTTTGAGGAAAAATAAAAATATAGATAATTTCCTAAATAGATA
TGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCAGAAGACT
ATGATGTTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTG
GTTCGTCATAAGGCATCACAGAAGGTTTATGCAATGAAGCTTCTTAGTAA
ATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAAAGAG
ATATTATGGCTTTTGCTAACAGTCCGTGGGTGGTTCAGCTCTTTTGTGCC
TTTCAAGATGACAGGTATCTGTACATGGTGATGGAGTACATGCCAGGTGG
AGACCTTGTTAACCTGATGAGCAATTATGATGTGCCTGAGAAGTGGGCCA
AGTTTTATACTGCTGAAGTCGTGCTTGCACTGGATGCAATACACTCCATG
GGCTTAATACACAGAGATGTGAAGCCCGATAACATGCTATTGGATAAACA
CGGACATCTAAAATTAGCAGATTTTGGCACATGTATGAAAATGGATGAAA
CAGGCATGGTGCATTGTGATACAGCAGTTGGAACTCCAGACTACATATCA
CCTGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGACGAGAATG
TGATTGGTGGTCTGTAGGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACA
CTCCATTTTATGCAGATTCACTTGTAGGAACCTACAGCAAAATTATGGAT
CATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGC
GAAGAATCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAA
GAAATGGGGTAGAAGAAATCAAATCAGCATCCTTCTTTAAGAATGATCAG
TGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTGCCTGAGCT
CAGCAGTGACATAGACAGCAGCAACTTTGATGACATTGAGGATGACAAAG
GCGATGTAGAGACCTTCCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTG
CCTTTTATAGGATTTACCTACTTTAGAGAAAATTTGTTACTAAGTGATTC
TCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAA
GTCAGGAGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGT
GAGGTACAAGCCAAGGAGGAACTGGAACAGAAGTGCAAGTCTATTAATAC
TCGTCTAGAGAAAACAGCAAAGGAACTAGAAGAAGAGATTACCTTTAGGA
AAAATGTGGAATCAACATTAAGACAGCTAGAAAGAGAAAAGGCCCTTCTT
CAGCACAAAAATGCAGAATATCAACGGAAAGCTGATCATGAAGCTGACAA
GAAACGGAATTTGGAAAATGATGTTAACAGCTTAAAAGATCAACTTGAAG
ATTTGAAGAAAAGAAACCAGAGCTCTCAGATATAAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGAPEAAPRDGAGAGRQRK
LEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIV
KKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMI
KRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVN
LMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLK
LADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWS
VGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLI
CAFLTDREVRLGRNGVEEIKSASFFKNDQWNWDNIRETAAPVVPELSSDI
DSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCR
ENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEK
TAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNL
ENDVNSLKDQLEDLKKRNQSSQI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3411
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GSTtag for baculovirus expression.