cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pET28a HA GFP 14-3-3-zeta
• Parent Plasmid: pET28a original
• Expressed: 6His-HA GFP 14-3-3-zeta
• Genbank: NM_145690
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGTGAGCAA
GGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACG
GCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGAT
GCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCT
GCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGT
GCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCC
GCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGA
CGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGG
TGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATC
CTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCAT
GGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACA
ACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACC
CCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCAC
CCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCC
TGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTG
TACAAGGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGC
TGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAG
GAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAA
AATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTATTGA
ACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACA
GAGAGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGTACTGTCTCTT
TTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGTCTT
CTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTGCCG
CTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATACCAA
GAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTATCAG
ACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGAACT
CCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCCATT
GCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCTAAT
AATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCCAAG
GAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAGGATCC

Amino Acid Sequence

MGSSLHHHHHSSGLVPRGSHMASMTGGQQMGRGSATMYPYDVPDYAMVSK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKL
PVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM
ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST
QSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKDKNELVQKAKLAEQA
ERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIE
QKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKV
FYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPI
RLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTL
IMQLLRDNLTLWTSDTQGDEAEAGEGGEN*

• Vector Type: Bacterial
• DU Number: DU3270
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/HA/GFP for bacterial expression
• Low Temp: No