cDNA Clone VPS34

• Plasmid: pGEX6P-1-HA VPS34
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA VPS34
• Genbank: NP_002638
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGAAGC
AGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGC
TTAAGATAGGAAGCTTGGAAGGGAAGAGAGAACAAAAGAGTTATAAAGCT
GTCCTGGAAGACCCAATGTTGAAGTTCTCAGGACTATATCAAGAGACATG
CTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTGG
CCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGG
AATGAATGGCTGAAACTACCAGTAAAATACCCTGACCTGCCCAGGAATGC
CCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGGAAAAGCAGTGC
CTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGC
CAAGGGATGCATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATC
AGAACCCACAAAAACTCCTGGCAGAACAAGTAGCACTCTCTCAGAAGATC
AGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACATG
GTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGAT
AAATGAGAGTGAAAAACGAAGTTCTAATTTCATGTACCTGATGGTTGAAT
TTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTGTTTATTATGAA
AAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAA
AGTTCCTGACCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACC
ACAAGCTTGCCCGGAGTTTAAGAAGTGGACCTTCTGACCACGATCTGAAA
CCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCACC
AACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGAT
ATTATCTTACGAATCAAGAAAAAGCCTTGACAAAATTCTTGAAATGTGTT
AATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAACTTCTGGGAAA
ATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATT
ACACCAACCCAACTGTGAGGCGTTATGCTGTTGCCCGGTTGCGACAGGCC
GATGATGAGGATTTGTTGATGTACCTATTACAATTGGTCCAGGCTCTCAA
ATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAGG
ATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCT
GCAGAAATAGATAGCTCCCAAATTATAACCAGCCCCCTTCCTTCAGTCTC
TTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGATGGCGAAAATC
TGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCA
ACACTGGCTAATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCA
AGATACTCAGCAGAGAGATCCAAAGACCCATGAGATGTACTTGAACGTAA
TGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGTT
ATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCA
TCTAATGAAGGCAGTACAACGCGAAAGTGGAAATCGTAAGAAAAAGAATG
AGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGAATTTGTCAGAT
GTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAAT
AATTCCGGAAACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGT
TGTTTTTTAAGACGGAAGATGGAGGCAAATATCCAGTTATATTTAAGCAT
GGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCAT
GGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATA
AGGTGTTAGCCACCAGTACAAAACATGGCTTCATGCAGTTTATCCAGTCA
GTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACTTTTT
TAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGG
TCATGGACACTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTAT
ATACTTGGAGTTGGAGACAGGCACCTGGATAACCTTTTGCTAACAAAAAC
AGGCAAACTCTTCCACATAGACTTTGGATATATTTTGGGTCGGGATCCAA
AGCCTCTTCCTCCACCAATGAAGCTGAATAAAGAAATGGTAGAAGGAATG
GGGGGCACACAGAGTGAGCAGTACCAAGAGTTCCGTAAACAGTGTTACAC
GGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGATTTTGAACTTGTTTT
CCTTGATGGTTGATGCAAACATTCCAGATATTGCACTTGAACCAGATAAA
ACTGTGAAAAAGGTTCAGGATAAATTCCGCTTAGACCTGTCGGATGAAGA
GGCTGTGCATTACATGCAGAGTCTGATTGATGAGAGTGTCCATGCTCTTT
TTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCCCAGTACTGGAGAAAA
TGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFATMYPYDVPDYAGEAE
KFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCS
DLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQ
VALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSE
PTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMIN
ESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKV
PDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPT
KQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKW
KPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKY
ENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSS
PPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQD
TQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHL
MKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGII
PETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMD
KLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFR
KYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTG
KLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTA
FLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEA
VHYMQSLIDESVHALFAAVVEQIHKFAQYWRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3202
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression.