cDNA Clone CRAF

• Synonyms: CRAF, RAF1
• Plasmid: pFastbac GST CRAF
• Parent Plasmid: pFastbac GST
• Expressed: GST 3C CRAF
• Genbank: BC018119
• Tag: GST
• Species: Human

Sequence of Insert

ggatccgagcacatacagggagcttggaagacgatcagcaatggttttgg
attcaaagatgccgtgtttgatggctccagctgcatctctcctacaatag
ttcagcagtttggctatcagcgccgggcatcagatgatggcaaactcaca
gatccttctaagacaagcaacactatccgtgttttcttgccgaacaagca
aagaacagtggtcaatgtgcgaaatggaatgagcttgcatgactgcctta
tgaaagcactcaaggtgaggggcctgcaaccagagtgctgtgcagtgttc
agacttctccacgaacacaaaggtaaaaaagcacgcttagattggaatac
tgatgctgcgtctttgattggagaagaacttcaagtagatttcctggatc
atgttcccctcacaacacacaactttgctcggaagacgttcctgaagctt
gccttctgtgacatctgtcagaaattcctgctcaatggatttcgatgtca
gacttgtggctacaaatttcatgagcactgtagcaccaaagtacctacta
tgtgtgtggactggagtaacatcagacaactcttattgtttccaaattcc
actattggtgatagtggagtcccagcactaccttctttgactatgcgtcg
tatgcgagagtctgtttccaggatgcctgttagttctcagcacagatatt
ctacacctcacgccttcacctttaacacctccagtccctcatctgaaggt
tccctctcccagaggcagaggtcgacatccacacctaatgtccacatggt
cagcaccaccctgcctgtggacagcaggatgattgaggatgcaattcgaa
gtcacagcgaatcagcctcaccttcagccctgtccagtagccccaacaat
ctgagcccaacaggctggtcacagccgaaaacccccgtgccagcacaaag
agagcgggcaccagtatctgggacccaggagaaaaacaaaattaggcctc
gtggacagagagattcaagctattattgggaaatagaagccagtgaagtg
atgctgtccactcggattgggtcaggctcttttggaactgtttataaggg
taaatggcacggagatgttgcagtaaagatcctaaaggttgtcgacccaa
ccccagagcaattccaggccttcaggaatgaggtggctgttctgcgcaaa
acacggcatgtgaacattctgcttttcatggggtacatgacaaaggacaa
cctggcaattgtgacccagtggtgcgagggcagcagcctctacaaacacc
tgcatgtccaggagaccaagtttcagatgttccagctaattgacattgcc
cggcagacggctcagggaatggactatttgcatgcaaagaacatcatcca
tagagacatgaaatccaacaatatatttctccatgaaggcttaacagtga
aaattggagattttggtttggcaacagtaaagtcacgctggagtggttct
cagcaggttgaacaacctactggctctgtcctctggatggccccagaggt
gatccgaatgcaggataacaacccattcagtttccagtcggatgtctact
cctatggcatcgtattgtatgaactgatgacgggggagcttccttattct
cacatcaacaaccgagatcagatcatcttcatggtgggccgaggatatgc
ctccccagatcttagtaagctatataagaactgccccaaagcaatgaaga
ggctggtagctgactgtgtgaagaaagtaaaggaagagaggcctcttttt
ccccagatcctgtcttccattgagctgctccaacactctctaccgaagat
caaccggagcgcttccgagccatccttgcatcgggcagcccacactgagg
atatcaatgcttgcacgctgaccacgtccccgaggctgcctgtcttctag
gaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEHIQGAWKTISNGFGFKDA
VFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVV
NVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAAS
LIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGY
KFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRES
VSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTL
PVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAP
VSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHG
DVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIV
TQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMK
SNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQ
DNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDL
SKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSA
SEPSLHRAAHTEDINACTLTTSPRLPVF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbac GST CRAF150.98 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1897
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion
• Low Temp: No