cDNA Clone GCKR

• Synonyms: MAP4K5
• Plasmid: pGEX6P-1-GCKR 300-480
• Parent Plasmid: pGEX6P1
• Expressed: GST-GCKR 300-480
• Genbank: NP_006566
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCACGCACATTACACTGAAGCAGATGACGATGACTTTGAGCCCCA
TGCAATCATTCGTCATACCATTAGATCTACAAACAGGAATGCCAGAGCTG
AACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCT
CTGAGAAAAGAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCC
AAATTTCATGTTACAGTGGAATCCTTTTGTTGATGGTGCAAATACTGGCA
AATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAGG
ATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAAC
CATAAAACATTGTCCTGATTCAGAAAGCAGAGCTCCCCAAATTCTCAGAA
GACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTTCTTCTATTGGA
AATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGC
ACAAGCACCACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAAT
AAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHAHYTEADDDDFEPHAIIR
HTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGLSSDPNFML
QWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHC
PDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQ
LPRKKDKRDFPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1896
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.