cDNA Clone MYPT1

• Plasmid: pGEX-MYPT1 377-976
• Parent Plasmid: pGEX4T1
• Expressed: GST-MYPT1 377-976
• Genbank: NM_053890
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCCCGCGGTATATTTCCGGTGGTGGTGGTGGAATTCTAGACTCCAT
GGCTTCTGTAACTAATGCTCACACTGCCAGCACTCAGGCAGCTCCTGCCG
CTGTGACAACACCTACTCTGTCTTCCAACCAGGGGACCCCTACATCACCT
GTTAAAAAGTTTCCTACATCAACTACAAAAATTTCTCCCAAAGAAGAAGA
AAGAAAAGATGAATCTCCTGCATCCTGGAGGTTAGGACTTAGAAAGACTG
GCAGTTATGGTGCCCTGGCTGAGATCACTGCATCTAAAGAAGCTCAGAAG
GAGAAAGACACTGCAGGCGTGATACGTTCAGCTTCGAGTCCCAGACTCTC
GTCCTCTTTGGATAATAAAGAAAAGGAGAAAGACAATAAAGGAACAAGAC
TTGCATATGTCGCCCCTACAATCCCAAGGCGACTAGGCAGTACGTCTGAC
ATTGAAGAGAAGGAAAACAGAGAGTCTTCAAATTTGCGAACAAGTAGTTC
TTACACAAGAAGAAAATGGGAAGATGATCTTAAAAAAAATAGTTCAATCA
ATGAAGGATCTACTTACCATAGAAGTACCTCAAATCGTTTGTGGGCTGAG
GATAGTACTGAGAAAGAGAAGGACAGTGCTCCTACCGCAGCGACCATTCT
TGTTGCTCCAACTGTTGTAAGTGCTGCAGCTTCTTCTACCACAGCCCTGA
CCACAACTACTGCTGGCACTCTTTCCTCCACATCAGAGGTCAGGGAGAGA
CGCAGGTCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAG
GAAAGCAAGATCTAGACAAGCAAGACAGTCTAGACGGTCAACACAGGGGG
TGACACTGACTGACCTCCAGGAAGCCGAAAAGACAATAGGAAGAAGCCGT
TCTACGAGAACCAGAGAACAAGAAAACGAAGAAAAAGACAAAGAAGAAAA
GGAAAAGCAGGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAGTAT
CTAGAGAAGATGAATATAAGCAAAAGTATTCCAGAACATACGATGAGACT
TATGCACGTTACAGACCAGTGTCAACTTCAAGTTCAAGCACTCCGTCGTC
CTCCTCACTTTCTACTCTAGGCAGTTCACTCTATGCCTCAAGTCAGCTCA
ACAGGCCAAACAGCCTTGTAGGTATAACCTCTGCCTACTCCCGGGGATTA
ACCAAAGACAATGAAAGAGAGGGAGAGAAAAAAGAAGAGGAAAAAGAAGG
GGAAGATAAGTCACAACCTAAATCAATCAGAGAACGACGGCGACCAAGAG
AAAAACGGAGGTCTACTGGAGTCTCCTTCTGGACACAAGATAGTGATGAA
AATGAGCAAGAGCGGCAGTCAGACACCGAGGATGGCTCCAGCAAGAGGGA
CACTCAGACGGATTCTGTTTCCAGGTATGACAGCAGTTCCACGTCATCAA
GCGATCGGTATGACTCCTTGCTGGGTCGTTCTGCCTCATACAGTTACTTA
GAAGAAAGGAAACCATATGGTAGCCGACTAGAAAAGGATGACTCAACTGA
CTTCAAAAAGCTTTATGAACAAATCTTAGCTGAAAATGAAAAACTAAAGG
CACAGCTACATGACACAAATATGGAACTAACGGATCTAAAGTTGCAGTTG
GAAAAAGCTACCCAGAGACAAGAACGATTTGCTGACAGGTCACTATTGGA
GATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAGATGG
AAGAGGAGCTCAAAATGTTACCAGACTTAAAAGCAGACAACCAGAGGCTA
AAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAGTA
GGACAGAAAACACACAAGCGAAGCAGCGGGACTTGCACACACTCCCCAGT
GGACCACATTGGCAGTCACTGGACGCCAGAAAGAACCCCTGGAGACTGTC
ATTTTCCGATATCAAGCTTATCGATACCGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPRYISGGGGGILDSMASVTNAHTA
STQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASW
RLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKE
KDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDD
LKKNSSINEGSTYHRSTSNRLWAEDSTEKEKDSAPTAATILVAPTVVSAA
ASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQ
SRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQE
EKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSS
LYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSI
RERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRY
DSSSTSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQIL
AENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRAL
ERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1887
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.