cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pEGFP-C1-6His 14-3-3-zeta
• Parent Plasmid: pEGFPC1
• Expressed: GFP-6His-14-3-3-zeta
• Genbank: NM_145690
• Tag: GFP
• Species: Human

Sequence of Insert

GGATATCCTCGCCAGAATTCGCCCATGGGACACCATCACCATCACCATCT
GGAAGTTCTGTTCCAGGGGCCCGATAAAAATGAGCTGGTTCAGAAGGCCA
AACTGGCCGAGCAGGCTGAGCGATATGATGACATGGCAGCCTGCATGAAG
TCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGAGGAATCTTCT
CTCAGTTGCTTATAAAAATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGG
TCGTCTCAAGTATTGAACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAG
ATGGCTCGAGAATACAGAGAGAAAATTGAGACGGAGCTAAGAGATATCTG
CAATGATGTACTGTCTCTTTTGGAAAAGTTCTTGATCCCCAATGCTTCAC
AAGCAGAGAGCAAAGTCTTCTATTTGAAAATGAAAGGAGATTACTACCGT
TACTTGGCTGAGGTTGCCGCTGGTGATGACAAGAAAGGGATTGTCGATCA
GTCACAACAAGCATACCAAGAAGCTTTTGAAATCAGCAAAAAGGAAATGC
AACCAACACATCCTATCAGACTGGGTCTGGCCCTTAACTTCTCTGTGTTC
TATTATGAGATTCTGAACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAGAC
AGCTTTTGATGAAGCCATTGCTGAACTTGATACATTAAGTGAAGAGTCAT
ACAAAGACAGCACGCTAATAATGCAATTACTGAGAGACAACTTGACATTG
TGGACATCGGATACCCAAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGA
AAATTAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGYPRQNSPMGHHHHHHLEVLFQGPDKNELVQKAKLAEQAER
YDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQK
TEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFY
LKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRL
GLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIM
QLLRDNLTLWTSDTQGDEAEAGEGGEN*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1882
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/6His tag for mammalian expression with precission protease site