cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pFB MSK§1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MSK1
• Genbank: O75582
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTGCCACCATGGACTACAAGGACGACGATGACAAGGAGGAGGAGGG
TGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAG
GAGAGCAGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACA
GGACATGCTGAGAAGGTGGGAATAGAAAATTTTGAGCTCCTGAAGGTCCT
AGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGTGGCC
ATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATC
GTTCAAAAGGCCAAAACCACAGAGCATACAAGGACAGAACGACAAGTCCT
GGAACACATTAGGCAGTCGCCATTTTTGGTAACATTACATTATGCTTTCC
AGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAA
CTTTTTACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCA
GATTTATGTTGGAGAGATTGTGCTTGCCCTCGAACATCTCCACAAGTTGG
GGATTATATATCGTGATATTAAGCTTGAGAATATTCTACTTGATTCTAAT
GGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAGGAGTTTGTGGCTGA
TGAAACTGAAAGAGCATATTCCTTTTGTGGAACTATTGAATACATGGCAC
CAGATATTGTCAGAGGGGGAGATTCAGGACATGACAAGGCAGTTGACTGG
TGGAGTTTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTT
CACTGTTGATGGAGAAAAAAATTCCCAAGCTGAGATATCTAGGAGAATAT
TAAAAAGTGAGCCTCCATATCCCCAAGAAATGAGTGCTTTAGCGAAAGAC
CTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAGAGATTGGGATGTGG
TCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAAATAA
ATTGGGATGATTTAGCCGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTC
ATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAAGAGTTCACAGAAAT
GGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGT
TTCAGGGCTATTCCTTTGTTGCTCCTTCCATCCTATTCAAGCGTAATGCA
GCTGTCATAGACCCTCTTCAGTTTCACATGGGAGTTGAACGTCCTGGAGT
GACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTATCAAC
ACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCA
ATTTGTCGAAAGTGTGTGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAA
AATAATCAGCAAAAGGATGGAAGCCAATACTCAAAAGGAAATAACAGCTC
TGAAACTCTGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTT
CATGATCAGCTTCACACGTTTCTAGTGATGGAACTTCTGAATGGAGGAGA
ACTGTTTGAGCGCATTAAGAAAAAGAAGCACTTCAGTGAGACGGAAGCCA
GCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGATGTT
GGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTATTGTTCACCGATGA
AAATGACAATTTGGAAATTAAAATAATTGATTTTGGATTTGCACGGCTAA
AGCCACCGGATAATCAGCCCCTGAAGACTCCATGCTTCACCCTTCATTAT
GCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGATGAGTCCTGTGACCT
GTGGAGCTTGGGCGTCATTTTGTACACAATGTTGTCAGGACAGGTTCCCT
TCCAATCTCATGACCGAAGTTTGACGTGTACCAGCGCGGTGGAAATCATG
AAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGAGAAGCCTGGAAGAA
TGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACTTCTCACAGTAGATC
CAAACAAAAGGCTTAAAATGTCTGGCTTGAGGTACAATGAATGGCTACAA
GATGGAAGTCAGCTGTCCTCCAATCCTCTGATGACTCCGGATATTCTAGG
ATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGCAACCTTCCACGCCT
TTAACAAATACAAGAGAGAGGGGTTTTGCCTTCAGAATGTTGATAAGGCC
CCTTTGGCTAAGAGAAGAAAAATGAAAAAGACTAGCACCAGTACCGAGAC
GCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTCCTCTCATTCTCACG
GTAAAACTACACCCACCAAGACACTGCAGCCCAGCAATCCTGCCGACAGC
AATAACCCGGAGACCCTCTTCCAGTTCTCGGACTCAGTAGCTTAGGCATG
GTAGGAGTGTATCAGTGATCCATTGCACCTTTATTCCCTCAGCATATGCC
TGAGGCGATTTTTTATGCTTTTAAAAATGTTTCCCGTTGGTTTCATTGGA
ATTTGCCTCCTAATGATTTTTTTTCAGGAAAACCTGTTTGGTTATCCTCA
TTCAAAAGCACTGGACAGAGAATGTTACTGTGAATAGAGCACATATTACT
TTTTTTAGCAACCTAGCATGATGCCAACAAGACTATTCTTGAAAGAGCAA
AGGTTCCTGTAAATTTAATTAGGGCTAGATTTGAGCTGCTTGTAAGTCAC
AGGTTTTCCAGATGTTTGCCAACAAGAAATGACTCATACTGTGATGATAC
CTTTTGCTTTGCCTTGTGGACAATGTGGGTTTTTGAAATTTGCACCCTTC
AAACAATGATTTATCAGAGAAAGGGGTCTGTTTTCAAAAAAGATTCTGTA
ATGAATTTTATGTGTGGCATATACTTATTTCTTGAGAGAAGATTTTAACT
TATTGTTTTTATTTTATGGTTACATATGATGATAACCTGCTATTATTAAA
CTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMDYKDDDDKEEEGGSSGGAA
GTSADGGDGGEQLLTVKHELRTANLTGHAEKVGIENFELLKVLGTGAYGK
VFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSP
FLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIV
LALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYS
FCGTIEYMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKN
SQAEISRRILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEI
KEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPA
ALPQSSEKLFQGYSFVAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSA
MMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRME
ANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKK
KKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIK
IIDFGFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVIL
YTMLSGQVPFQSHDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKD
LIQGLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMTPDILGSSGAAVH
TCVKATFHAFNKYKREGFCLQNVDKAPLAKRRKMKKTSTSTETRSSSSES
SHSSSSHSHGKTTPTKTLQPSNPADSNNPETLFQFSDSVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1849
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/FLAG tag for baculovirus expression