cDNA Clone GRB14

• Plasmid: pGEX6P-1-GRB14
• Parent Plasmid: pGEX6P1
• Expressed: GST-GRB14
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACTAGTACCACTTCCCTGCAAGATGGGCAGAGCGCCGCGAGCAG
GGCGGCTGCCCGGGATTCGCCGCTGGCCGCCCAGGTGTGTGGCGCTGCCC
AGGGGAGGGGCGACGCCCACGACCTGGCGCCGGCCCCCTGGCTGCACGCG
CGAGCGCTCCTGCCCCTTCCGGACGGGACCCGCGGCTGTGCTGCAGACAG
GAGAAAAAAGAAAGATCTTGATGTTCCGGAAATGCCATCTATTCCAAACC
CTTTTCCTGAGCTATGCTGTTCTCCAATTACATCTGTGTTGTCAGCAGAC
CTATTTCCCAAAGCAAATTCAAGGAAAAAACAGGTGATTAAAGTATACAG
TGAAGATGAAACCAGCAGGGCTTTAGATGTACCCAGTGACATAACGGCTC
GAGATGTTTGTCAGCTGTTGATCCTGAAGAATCATTACATTGATGACCAC
AGCTGGACCCTTTTTGAGCACCTGCCTCACATAGGTGTAGAAAGAACAAT
AGAAGACCACGAACTGGTGATTGAAGTGCTATCCAACTGGGGGATAGAAG
AAGAAAACAAACTATACTTTAGAAAAAATTATGCCAAATATGAGTTCTTT
AAAAACCCAATGTATTTTTTTCCAGAGCATATGGTATCTTTTGCAACTGA
AACCAATGGTGAAATATCCCCCACACAGATTTTGCAGATGTTTCTGAGTT
CAAGCACATATCCTGAAATTCATGGTTTCTTACATGCGAAAGAACAGGGA
AAGAAGTCTTGGAAAAAAATTTACTTTTTTCTAAGAAGATCTGGTTTATA
TTTTTCTACTAAAGGAACATCAAAGGAACCGCGGCATTTGCAGTTTTTCA
GCGAATTTGGCAATAGTGATATTTATGTGTCACTGGCAGGCAAAAAAAAA
CATGGAGCACCGACTAACTATGGATTCTGCTTTAAGCCTAACAAAGCGGG
AGGGCCCCGAGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGTAGGA
CGTGCTGGGTGACCGCGATTAGATTGCTTAAGTATGGCATGCAGCTGTAC
CAGAATTATATGCATCCATATCAAGGTAGAAGTGGCTGCAGTTCACAGAG
CATATCACCTATGAGAAGTATATCAGAGAATTCCCTGGTAGCAATGGACT
TCTCAGGCCAGAAAAGCAGAGTTATAGAAAATCCCACTGAAGCCCTTTCA
GTTGCGGTTGAAGAAGGACTCGCTTGGAGGAAAAAAGGATGTTTACGCCT
GGGCACTCACGGTAGCCCCACTGCCTCTTCACAGAGCTCTGCCACAAACA
TGGCTATCCACCGGTCCCAGCCATGGTTTCACCACAAAATTTCTAGAGAT
GAGGCTCAGCGATTGATTATTCAGCAAGGACTTGTGGATGGAGTTTTCTT
GGTACGGGATAGTCAGAGTAACCCCAAAACTTTCGTACTGTCAATGAGTC
ATGGACAAAAAATAAAGCACTTTCAAATTATACCAGTAGAAGATGACGGT
GAAATGTTCCACACACTGGATGATGGCCACACAAGATTTACAGATCTAAT
ACAGCTGGTGGAGTTCTATCAACTCAATAAGGGCGTTCTTCCTTGCAAGT
TGAAACATTATTGTGCTAGGATTGCTCTCTAGgtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTSTTSLQDGQSAASRAAAR
DSPLAAQVCGAAQGRGDAHDLAPAPWLHARALLPLPDGTRGCAADRRKKK
DLDVPEMPSIPNPFPELCCSPITSVLSADLFPKANSRKKQVIKVYSEDET
SRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGVERTIEDHE
LVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGE
ISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTK
GTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRD
LKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPM
RSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCLRLGTHG
SPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGVFLVRDS
QSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVE
FYQLNKGVLPCKLKHYCARIAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee