cDNA Clone G6PDH

• Plasmid: pEGFP-C1-HA-G6PDH
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HA G6PDH
• Genbank: NM_000402
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCAGAGCA
GGTGGCCCTGAGCCGGACCCAGGTGTGCGGGATCCTGCGGGAAGAGCTTT
TCCAGGGCGATGCCTTCCATCAGTCGGATACACACATATTCATCATCATG
GGTGCATCGGGTGACCTGGCCAAGAAGAAGATCTACCCCACCATCTGGTG
GCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCGTGGGCTATG
CCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTC
TTCAAGGCCACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCG
CAACTCCTATGTGGCTGGCCAGTACGATGATGCAGCCTCCTACCAGCGCC
TCAACAGCCACATGGATGCCCTCCACCTGGGGTCACAGGCCAACCGCCTC
TTCTACCTGGCCTTGCCCCCGACCGTCTACGAGGCCGTCACCAAGAACAT
TCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCATCGTGGAGA
AGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATC
TCCTCCCTGTTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGG
CAAGGAGATGGTGCAGAACCTCATGGTGCTGAGATTTGCCAACAGGATCT
TCGGCCCCATCTGGAACCGGGACAACATCGCCTGCGTTATCCTCACCTTC
AAGGAGCCCTTTGGCACTGAGGGTCGCGGGGGCTATTTCGATGAATTTGG
GATCATCCGGGACGTGATGCAGAACCACCTACTGCAGATGCTGTGTCTGG
TGGCCATGGAGAAGCCCGCCTCCACCAACTCAGATGACGTCCGTGATGAG
AAGGTCAAGGTGTTGAAATGCATCTCAGAGGTGCAGGCCAACAATGTGGT
CCTGGGCCAGTACGTGGGGAACCCCGATGGAGAGGGCGAGGCCACCAAAG
GGTACCTGGACGACCCCACGGTGCCCCGCGGGTCCACCACCGCCACTTTT
GCAGCCGTCGTCCTCTATGTGGAGAATGAGAGGTGGGATGGGGTGCCCTT
CATCCTGCGCTGCGGCAAGGCCCTGAACGAGCGCAAGGCCGAGGTGAGGC
TGCAGTTCCATGATGTGGCCGGCGACATCTTCCACCAGCAGTGCAAGCGC
AACGAGCTGGTGATCCGCGTGCAGCCCAACGAGGCCGTGTACACCAAGAT
GATGACCAAGAAGCCGGGCATGTTCTTCAACCCCGAGGAGTCGGAGCTGG
ACCTGACCTACGGCAACAGATACAAGAACGTGAAGCTCCCTGACGCCTAC
GAGCGCCTCATCCTGGACGTCTTCTGCGGGAGCCAGATGCACTTCGTGCG
CAGCGACGAGCTCCGTGAGGCCTGGCGTATTTTCACCCCACTGCTGCACC
AGATTGAGCTGGAGAAGCCCAAGCCCATCCCCTATATTTATGGCAGCCGA
GGCCCCACGGAGGCAGACGAGCTGATGAAGAGAGTGGGTTTCCAGTATGA
GGGCACCTACAAGTGGGTGAACCCCCACAAGCTCTGAGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
MYPYDVPDYAAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASG
DLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKAT
PEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMDALHLGSQANRLFYLA
LPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLF
REDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPF
GTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKV
LKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVV
LYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELV
IRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLI
LDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTE
ADELMKRVGFQYEGTYKWVNPHKL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct with N terminal HA tag