cDNA Clone G6PDH

• Plasmid: pGEX6P-1-HA G6PDH
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA G6PDH
• Genbank: NM_000402
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCAGAGCA
GGTGGCCCTGAGCCGGACCCAGGTGTGCGGGATCCTGCGGGAAGAGCTTT
TCCAGGGCGATGCCTTCCATCAGTCGGATACACACATATTCATCATCATG
GGTGCATCGGGTGACCTGGCCAAGAAGAAGATCTACCCCACCATCTGGTG
GCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCGTGGGCTATG
CCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTC
TTCAAGGCCACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCG
CAACTCCTATGTGGCTGGCCAGTACGATGATGCAGCCTCCTACCAGCGCC
TCAACAGCCACATGGATGCCCTCCACCTGGGGTCACAGGCCAACCGCCTC
TTCTACCTGGCCTTGCCCCCGACCGTCTACGAGGCCGTCACCAAGAACAT
TCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCATCGTGGAGA
AGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATC
TCCTCCCTGTTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGG
CAAGGAGATGGTGCAGAACCTCATGGTGCTGAGATTTGCCAACAGGATCT
TCGGCCCCATCTGGAACCGGGACAACATCGCCTGCGTTATCCTCACCTTC
AAGGAGCCCTTTGGCACTGAGGGTCGCGGGGGCTATTTCGATGAATTTGG
GATCATCCGGGACGTGATGCAGAACCACCTACTGCAGATGCTGTGTCTGG
TGGCCATGGAGAAGCCCGCCTCCACCAACTCAGATGACGTCCGTGATGAG
AAGGTCAAGGTGTTGAAATGCATCTCAGAGGTGCAGGCCAACAATGTGGT
CCTGGGCCAGTACGTGGGGAACCCCGATGGAGAGGGCGAGGCCACCAAAG
GGTACCTGGACGACCCCACGGTGCCCCGCGGGTCCACCACCGCCACTTTT
GCAGCCGTCGTCCTCTATGTGGAGAATGAGAGGTGGGATGGGGTGCCCTT
CATCCTGCGCTGCGGCAAGGCCCTGAACGAGCGCAAGGCCGAGGTGAGGC
TGCAGTTCCATGATGTGGCCGGCGACATCTTCCACCAGCAGTGCAAGCGC
AACGAGCTGGTGATCCGCGTGCAGCCCAACGAGGCCGTGTACACCAAGAT
GATGACCAAGAAGCCGGGCATGTTCTTCAACCCCGAGGAGTCGGAGCTGG
ACCTGACCTACGGCAACAGATACAAGAACGTGAAGCTCCCTGACGCCTAC
GAGCGCCTCATCCTGGACGTCTTCTGCGGGAGCCAGATGCACTTCGTGCG
CAGCGACGAGCTCCGTGAGGCCTGGCGTATTTTCACCCCACTGCTGCACC
AGATTGAGCTGGAGAAGCCCAAGCCCATCCCCTATATTTATGGCAGCCGA
GGCCCCACGGAGGCAGACGAGCTGATGAAGAGAGTGGGTTTCCAGTATGA
GGGCACCTACAAGTGGGTGAACCCCCACAAGCTCTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFATMYPYDVPDYAAEQV
ALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWL
FRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARN
SYVAGQYDDAASYQRLNSHMDALHLGSQANRLFYLALPPTVYEAVTKNIH
ESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGK
EMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGI
IRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVL
GQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFI
LRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMM
TKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRS
DELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEG
TYKWVNPHKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1655
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal GST/HA tag