cDNA Clone SHIP2

• Plasmid: pFBHTb SHIP2 18-pFBHTb
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-SHIP2 18-HSY14385
• Tag: 6His
• Species: Human

Sequence of Insert

GCACTAGTGGATCCATGGCCCCCTCCTGGTACCACCGCGACCTGAGCCGG
GCGGCCGCGGAGGAGCTGCTGGCCCGGGCGGGCCGCGATGGCAGCTTCCT
GGTCCGAGACAGCGAGAGCGTGGCGGGGGCCTTCGCGCTCTGCGTCCTGT
ATCAGAAGCATGTGCACACGTATCGCATTCTGCCTGATGGAGAAGATTTC
TTGGCTGTGCAGACCTCGCAGGGTGTGCCTGTGCGCCGCTTCCAGACCCT
GGGTGAGCTCATCGGCCTGTACGCCCAGCCCAACCAGGGCCTTGTGTGCG
CCCTGCTTCTTCCTGTAGAGGGTGAGCGAGAGCCGGACCCACCGGATGAC
CGGGATGCCTCAGATGGGGAGGATGAGAAGCCCCCGCTGCCCCCGCGCTC
TGGCTCCACCAGCATTTCTGCCCCCACTGGGCCCAGCAGTCCCCTGCCAG
CTCCTGAGACTCCCACAGCTCCAGCTGCTGAGAGTGCTCCCAATGGGCTG
AGCACCGTCTCGCACGACTACCTGAAAGGCAGCTATGGGCTGGACCTGGA
AGCTGTGAGGGGTGGAGCCAGCCACCTGCCCCACCTCACCCGTACCCTCG
CTACCTCATGCCGGAGGCTGCACAGTGAGGTGGACAAGGTCCTGTCAGGC
CTGGAGATCCTGTCCAAGGTGTTTGACCAGCAGAGCTCGCCCATGGTGAC
CCGCCTTTTGCAGCAGCAGAACCTGCCACAGACAGGGGAGCAGGAACTAG
AGAGCCTGGTGCTGAAGCTGTCAGTGCTAAAGGACTTCCTGTCAGGCATC
CAGAAGAAGGCCCTGAAGGCCCTACAGGACATGAGCTCCACAGCACCCCC
AGCTCCGCAGCCATCCACACGTAAGGCCAAGACCATCCCCGTGCAGGCCT
TTGAGGTGAAGCTAGATGTGACCCTGGGTGACCTGACCAAGATTGGGAAG
TCACAGAAGTTCACGCTGAGCGTGGATGTGGAGGGTGGGCGGCTGGTGCT
GCTGCGGAGACAGCGGGACTCCCAGGAGGACTGGACCACCTTCACGCACG
ACCGCATCCGCCAGCTCATTAAGTCCCAGCGTGTCCAGAACAAGCTGGGT
GTTGTGTTTGAGAAGGAGAAGGACCGGACTCAGCGCAAGGACTTCATCTT
TGTCAGTGCCCGGAAGCGGGAGGCCTTCTGCCAGCTGTTGCAGCTCATGA
AGAACAAGCACTCCAAGCAGGACGAGCCCGACATGATCTCAGTCTTCATA
GGCACCTGGAACATGGGAAGTGTACCACCTCCAAAAAACGTGACATCCTG
GTTCACATCGAAGGGTCTGGGGAAGACCCTGGACGAGGTCACAGTGACCA
TACCCCATGACATCTATGTCTTTGGGACCCAGGAGAACTCAGTGGGCGAC
CGCGAGTGGCTGGACCTACTGCGCGGGGGCCTCAAGGAGCTTACGGATCT
GGATTACCGCCCGATTGCCATGCAATCACTGTGGAATATCAAGGTGGCAG
TGCTGGTCAAGCCAGAGCACGAGAACCGTATCAGCCATGTCAGTACGTCC
AGTGTGAAGACTGGCATCGCCAACACCCTGGGGAACAAGGGGGCTGTGGG
CGTCTCCTTCATGTTTAATGGCACCTCATTTGGCTTTGTGAATTGTCACC
TCACCTCGGGAAATGAGAAGACGGCTCGGAGGAACCAAAACTACTTGGAC
ATCCTGCGGCTGCTCTCGCTGGGCGACCGGCAGCTCAATGCCTTTGACAT
CTCTCTGCGTTTCACACACCTCTTCTGGTTTGGGGACCTCAACTACCGCC
TGGACATGGATATCCAGGAGATCCTGAACTACATCAGCAGGAAAGAGTTT
GAGCCCCTCCTCAGGGTGGACCAGCTCAACCTGGAGCGGGAGAAGCACAA
GGTCTTCCTTCGATTCAGTGAGGAGGAGATCTCCTTCCCACCCACCTACC
GCTATGAGCGGGGTTCCCGGGACACATATGCCTGGCACAAGCAGAAGCCA
ACTGGGGTCCGGACCAATGTGCCCTCATGGTGTGACCGGATTCTGTGGAA
ATCCTACCCTGAAACTCACATCATCTGCAATTCTTATGGTTGCACTGATG
ACATCGTCACCAGCGACCATTCCCCCGTGTTTGGGACATTTGAGGTTGGA
GTTACCTCCCAGTTCATCTCCAAGAAAGGGCTCTCAAAGACTTCAGACCA
GGCCTACATTGAGTTTGAGAGCATCGAGGCCATTGTGAAGACAGCCAGCC
GCACCAAGTTCTTCATCGAGTTCTACTCTACCTGCCTGGAGGAATACAAG
AAGAGCTTTGAGAATGATGCCCAGAGCAGTGACAACATCAACTTCCTCAA
AGTGCAGTGGTCTTCACGCCAGCTGCCCACGCTCAAACCAATTCTGGCTG
ATATCGAGTACCTGCAGGACCAGCACCTCCTGCTCACAGTCAAGTCCATG
GATGGCTATGAATCCTATGGGGAGTGTGTGGTTGCACTCAAATCCATGAT
CGGCAGCACGGCCCAACAGTTCCTGACCTTCCTATCCCACCGTGGCGAGG
AGACAGGCAATATCAGAGGCTCCATGAAGGTGCGGGTGCCCACGGAGCGC
CTGGGCACCCGTGAGCGGCTCTACGAGTGGATCAGCATTGATAAGGATGA
GGCAGGAGCAAAGAGCAAAGCCCCCTCTGTGTCCCGAGGGAGCCAGGAGC
CCAGGTCAGGGAGCCGCAAGCCAGCCTTCACAGAGGCCTCCTGCCCGCTC
TCCAGGTTATTTGAAGAACCAGAGAAACCGCCACCAACGGGGAGGCCCCC
AGCCCCACCCCGAGCAGCTCCCCGGGAGGAGCCCTTGACCCCCAGGTTGA
AGCCAGAGGGAGCTCCTGAACCAGAAGGGGTGGCGGCCCCCCCACCCAAG
AACAGCTTCAATAACCCTGCCTGAACTAGTGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAPSWYHRDLSRAAAEELLARA
GRDGSFLVRDSESVAGAFALCVLYQKHVHTYRILPDGEDFLAVQTSQGVP
VRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASDGEDEK
PPLPPRSGSTSISAPTGPSSPLPAPETPTAPAAESAPNGLSTVSHDYLKG
SYGLDLEAVRGGASHLPHLTRTLATSCRRLHSEVDKVLSGLEILSKVFDQ
QSSPMVTRLLQQQNLPQTGEQELESLVLKLSVLKDFLSGIQKKALKALQD
MSSTAPPAPQPSTRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFTLSVDV
EGGRLVLLRRQRDSQEDWTTFTHDRIRQLIKSQRVQNKLGVVFEKEKDRT
QRKDFIFVSARKREAFCQLLQLMKNKHSKQDEPDMISVFIGTWNMGSVPP
PKNVTSWFTSKGLGKTLDEVTVTIPHDIYVFGTQENSVGDREWLDLLRGG
LKELTDLDYRPIAMQSLWNIKVAVLVKPEHENRISHVSTSSVKTGIANTL
GNKGAVGVSFMFNGTSFGFVNCHLTSGNEKTARRNQNYLDILRLLSLGDR
QLNAFDISLRFTHLFWFGDLNYRLDMDIQEILNYISRKEFEPLLRVDQLN
LEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTGVRTNVPSW
CDRILWKSYPETHIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQFISKKG
LSKTSDQAYIEFESIEAIVKTASRTKFFIEFYSTCLEEYKKSFENDAQSS
DNINFLKVQWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYESYGECV
VALKSMIGSTAQQFLTFLSHRGEETGNIRGSMKVRVPTERLGTRERLYEW
ISIDKDEAGAKSKAPSVSRGSQEPRSGSRKPAFTEASCPLSRLFEEPEKP
PPTGRPPAPPRAAPREEPLTPRLKPEGAPEPEGVAAPPPKNSFNNPA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1651
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag