cDNA Clone DAP

• Plasmid: pCMV5-HA DAP
• Parent Plasmid: pCMV5
• Expressed: HA-DAP
• Genbank: NP_036232
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGGTGGC
CATGAACGGTAAGGCCCGCAAAGAGGCGGTGCAGACTGCGGCTAAGGAAC
TCCTCAAGTTCGTGAACCGGAGTCCCTCTCCTTTCCATGCTGTGGCTGAA
TGCCGCAACCGCCTTCTCCAGGCTGGCTTCAGTGAACTCAAGGAGACTGA
GAAATGGAATATTAAGCCCGAGAGCAAGTACTTCATGACCAGGAACTCCT
CCACCATCATAGCTTTTGCTGTAGGGGGCCAGTACGTTCCTGGCAATGGC
TTCAGCCTCATCGGGGCCCACACGGACAGCCCCTGCCTCCGGGTGAAACG
TCGGTCTCGCCGCAGCCAGGTGGGCTTCCAGCAAGTCGGTGTGGAGACCT
ATGGTGGTGGGATCTGGAGCACCTGGTTTGACCGTGACCTGACTCTGGCT
GGACGCGTCATTGTCAAGTGCCCTACCTCAGGTCGGCTGGAGCAGCAGCT
GGTGCACGTGGAGCGGCCCATTCTTCGCATCCCACACCTGGCCATCCATC
TGCAGCGAAATATCAACGAGAACTTTGGGCCCAACACAGAGATGCATCTA
GTCCCCATTCTTGCCACAGCCATCCAGGAGGAGCTGGAGAAGGGGACTCC
TGAGCCAGGGCCTCTCAATGCTGTGGATGAGCGGCACCATTCGGTCCTCA
TGTCCCTGCTCTGTGCCCATCTGGGGCTGAGCCCCAAGGACATAGTGGAG
ATGGAGCTCTGCCTTGCAGACACCCAGCCTGCGGTCTTGGGTGGTGCCTA
TGATGAGTTCATCTTTGCTCCTCGGCTGGACAATCTGCACAGCTGCTTCT
GTGCCCTGCAGGCCTTGATAGATTCCTGTGCAGGCCCTGGCTCCCTGGCC
ACAGAGCCTCACGTGCGCATGGTCACACTCTATGACAACGAAGAGGTGGG
GTCTGAGAGTGCACAGGGAGCACAGTCACTGCTGACAGAGCTGGTGCTGC
GGCGGATCTCAGCCTCGTGCCAGCACCCGACAGCCTTCGAGGAAGCCATA
CCCAAGTCCTTCATGATCAGCGCAGACATGGCCCATGCTGTGCATCCCAA
CTACCTGGACAAGCATGAGGAGAACCACCGGCCTTTATTCCACAAGGGCC
CCGTGATCAAGGTGAACAGCAAGCAACGCTATGCTTCAAACGCGGTGTCA
GAGGCCCTGATCCGAGAGGTGGCCAACAAAGTCAAGGTCCCCCTGCAGGA
TCTCATGGTCCGGAATGACACCCCCTGTGGAACCACCATTGGACCTATCT
TGGCTTCTCGGCTGGGGCTGCGGGTGCTGGATTTAGGCAGCCCCCAACTG
GCCATGCACTCTATCCGGGAGATGGCCTGCACCACAGGAGTCCTCCAGAC
CCTCACCCTCTTCAAGGGCTTCTTTGAGCTGTTCCCTTCTCTAAGCCATA
ATCTCTTAGTGGATTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAQVAMNGKARKEAVQTAAKELLKFVNRSPSPFHAVAECRNR
LLQAGFSELKETEKWNIKPESKYFMTRNSSTIIAFAVGGQYVPGNGFSLI
GAHTDSPCLRVKRRSRRSQVGFQQVGVETYGGGIWSTWFDRDLTLAGRVI
VKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINENFGPNTEMHLVPIL
ATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHLGLSPKDIVEMELC
LADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALIDSCAGPGSLATEPH
VRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQHPTAFEEAIPKSF
MISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNAVSEALI
REVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAMHS
IREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1644
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression