cDNA Clone DAP

• Plasmid: pEGFP-C1-HA-DAP
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HA DAP
• Genbank: NP_036232
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGGTGGC
CATGAACGGTAAGGCCCGCAAAGAGGCGGTGCAGACTGCGGCTAAGGAAC
TCCTCAAGTTCGTGAACCGGAGTCCCTCTCCTTTCCATGCTGTGGCTGAA
TGCCGCAACCGCCTTCTCCAGGCTGGCTTCAGTGAACTCAAGGAGACTGA
GAAATGGAATATTAAGCCCGAGAGCAAGTACTTCATGACCAGGAACTCCT
CCACCATCATAGCTTTTGCTGTAGGGGGCCAGTACGTTCCTGGCAATGGC
TTCAGCCTCATCGGGGCCCACACGGACAGCCCCTGCCTCCGGGTGAAACG
TCGGTCTCGCCGCAGCCAGGTGGGCTTCCAGCAAGTCGGTGTGGAGACCT
ATGGTGGTGGGATCTGGAGCACCTGGTTTGACCGTGACCTGACTCTGGCT
GGACGCGTCATTGTCAAGTGCCCTACCTCAGGTCGGCTGGAGCAGCAGCT
GGTGCACGTGGAGCGGCCCATTCTTCGCATCCCACACCTGGCCATCCATC
TGCAGCGAAATATCAACGAGAACTTTGGGCCCAACACAGAGATGCATCTA
GTCCCCATTCTTGCCACAGCCATCCAGGAGGAGCTGGAGAAGGGGACTCC
TGAGCCAGGGCCTCTCAATGCTGTGGATGAGCGGCACCATTCGGTCCTCA
TGTCCCTGCTCTGTGCCCATCTGGGGCTGAGCCCCAAGGACATAGTGGAG
ATGGAGCTCTGCCTTGCAGACACCCAGCCTGCGGTCTTGGGTGGTGCCTA
TGATGAGTTCATCTTTGCTCCTCGGCTGGACAATCTGCACAGCTGCTTCT
GTGCCCTGCAGGCCTTGATAGATTCCTGTGCAGGCCCTGGCTCCCTGGCC
ACAGAGCCTCACGTGCGCATGGTCACACTCTATGACAACGAAGAGGTGGG
GTCTGAGAGTGCACAGGGAGCACAGTCACTGCTGACAGAGCTGGTGCTGC
GGCGGATCTCAGCCTCGTGCCAGCACCCGACAGCCTTCGAGGAAGCCATA
CCCAAGTCCTTCATGATCAGCGCAGACATGGCCCATGCTGTGCATCCCAA
CTACCTGGACAAGCATGAGGAGAACCACCGGCCTTTATTCCACAAGGGCC
CCGTGATCAAGGTGAACAGCAAGCAACGCTATGCTTCAAACGCGGTGTCA
GAGGCCCTGATCCGAGAGGTGGCCAACAAAGTCAAGGTCCCCCTGCAGGA
TCTCATGGTCCGGAATGACACCCCCTGTGGAACCACCATTGGACCTATCT
TGGCTTCTCGGCTGGGGCTGCGGGTGCTGGATTTAGGCAGCCCCCAACTG
GCCATGCACTCTATCCGGGAGATGGCCTGCACCACAGGAGTCCTCCAGAC
CCTCACCCTCTTCAAGGGCTTCTTTGAGCTGTTCCCTTCTCTAAGCCATA
ATCTCTTAGTGGATTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMYPYDVPDYAQVAMNGKARKEAVQTAAKELLKFVNRS
PSPFHAVAECRNRLLQAGFSELKETEKWNIKPESKYFMTRNSSTIIAFAV
GGQYVPGNGFSLIGAHTDSPCLRVKRRSRRSQVGFQQVGVETYGGGIWST
WFDRDLTLAGRVIVKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINEN
FGPNTEMHLVPILATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHL
GLSPKDIVEMELCLADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALID
SCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQ
HPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSK
QRYASNAVSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLR
VLDLGSPQLAMHSIREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1643
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFT/HA tag for mammalian expression