cDNA Clone DAP

• Plasmid: pGEX6P-1-HA DAP
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA DAP
• Genbank: NP_036232
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGGTGGC
CATGAACGGTAAGGCCCGCAAAGAGGCGGTGCAGACTGCGGCTAAGGAAC
TCCTCAAGTTCGTGAACCGGAGTCCCTCTCCTTTCCATGCTGTGGCTGAA
TGCCGCAACCGCCTTCTCCAGGCTGGCTTCAGTGAACTCAAGGAGACTGA
GAAATGGAATATTAAGCCCGAGAGCAAGTACTTCATGACCAGGAACTCCT
CCACCATCATAGCTTTTGCTGTAGGGGGCCAGTACGTTCCTGGCAATGGC
TTCAGCCTCATCGGGGCCCACACGGACAGCCCCTGCCTCCGGGTGAAACG
TCGGTCTCGCCGCAGCCAGGTGGGCTTCCAGCAAGTCGGTGTGGAGACCT
ATGGTGGTGGGATCTGGAGCACCTGGTTTGACCGTGACCTGACTCTGGCT
GGACGCGTCATTGTCAAGTGCCCTACCTCAGGTCGGCTGGAGCAGCAGCT
GGTGCACGTGGAGCGGCCCATTCTTCGCATCCCACACCTGGCCATCCATC
TGCAGCGAAATATCAACGAGAACTTTGGGCCCAACACAGAGATGCATCTA
GTCCCCATTCTTGCCACAGCCATCCAGGAGGAGCTGGAGAAGGGGACTCC
TGAGCCAGGGCCTCTCAATGCTGTGGATGAGCGGCACCATTCGGTCCTCA
TGTCCCTGCTCTGTGCCCATCTGGGGCTGAGCCCCAAGGACATAGTGGAG
ATGGAGCTCTGCCTTGCAGACACCCAGCCTGCGGTCTTGGGTGGTGCCTA
TGATGAGTTCATCTTTGCTCCTCGGCTGGACAATCTGCACAGCTGCTTCT
GTGCCCTGCAGGCCTTGATAGATTCCTGTGCAGGCCCTGGCTCCCTGGCC
ACAGAGCCTCACGTGCGCATGGTCACACTCTATGACAACGAAGAGGTGGG
GTCTGAGAGTGCACAGGGAGCACAGTCACTGCTGACAGAGCTGGTGCTGC
GGCGGATCTCAGCCTCGTGCCAGCACCCGACAGCCTTCGAGGAAGCCATA
CCCAAGTCCTTCATGATCAGCGCAGACATGGCCCATGCTGTGCATCCCAA
CTACCTGGACAAGCATGAGGAGAACCACCGGCCTTTATTCCACAAGGGCC
CCGTGATCAAGGTGAACAGCAAGCAACGCTATGCTTCAAACGCGGTGTCA
GAGGCCCTGATCCGAGAGGTGGCCAACAAAGTCAAGGTCCCCCTGCAGGA
TCTCATGGTCCGGAATGACACCCCCTGTGGAACCACCATTGGACCTATCT
TGGCTTCTCGGCTGGGGCTGCGGGTGCTGGATTTAGGCAGCCCCCAACTG
GCCATGCACTCTATCCGGGAGATGGCCTGCACCACAGGAGTCCTCCAGAC
CCTCACCCTCTTCAAGGGCTTCTTTGAGCTGTTCCCTTCTCTAAGCCATA
ATCTCTTAGTGGATTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAQVAMNGK
ARKEAVQTAAKELLKFVNRSPSPFHAVAECRNRLLQAGFSELKETEKWNI
KPESKYFMTRNSSTIIAFAVGGQYVPGNGFSLIGAHTDSPCLRVKRRSRR
SQVGFQQVGVETYGGGIWSTWFDRDLTLAGRVIVKCPTSGRLEQQLVHVE
RPILRIPHLAIHLQRNINENFGPNTEMHLVPILATAIQEELEKGTPEPGP
LNAVDERHHSVLMSLLCAHLGLSPKDIVEMELCLADTQPAVLGGAYDEFI
FAPRLDNLHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESA
QGAQSLLTELVLRRISASCQHPTAFEEAIPKSFMISADMAHAVHPNYLDK
HEENHRPLFHKGPVIKVNSKQRYASNAVSEALIREVANKVKVPLQDLMVR
NDTPCGTTIGPILASRLGLRVLDLGSPQLAMHSIREMACTTGVLQTLTLF
KGFFELFPSLSHNLLVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1642
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression