cDNA Clone F2KP

• Plasmid: pGEX-At F2KP S303A
• Parent Plasmid: pGEX6P1
• Genbank: NM_100584
• Species: Arabidopsis thaliana

Sequence of Insert

GAATTCCCGGGTCGACGGGTCAGGTGCATCGAAGAATACTGAGGAAGATG
ACGATGGTTCCAATGGCGGAGGTGGTCAGCTCTACGTTTCACTCAAAATG
GAAAACTCTAAGGTTGAAGGAGAGCTCACTCCTCATGTCTATGGCTCTCT
TCCTCTCATCGGTTCCTGGGATCCTTCCAAAGCTCTTCCGATGCAACGTG
AATCTGCGTTAATGTCGGAGCTAAGCTTCGTTGTTCCTCCTGATCACGAG
ACTTTGGACTTCAAGTTTTTGTTGAAGCCAAAGAACAGAAATACGCCCTG
CATAGTGGAGGAGGGTGAAAATAGGCTTCTTACTGGTGGTTCGTTGCAAG
GAGATGCTAGATTAGCTCTGTTTAGGCTGGAAGGTGATGTGATTGTTGAA
TTCCGAGTCTTCATCAACGCTGACAGAGTTTCTCCAATTGATCTTGCTAC
TAGTTGGAGAGCTTATAGGGAGAACCTCCAGCCTTCTACTGTACGTGGAA
TTCCTGATGTCAGCATTAATCCTGATCCAAAATCTGCTGAGTGTCCTTTA
GAAAGTTTGGAGCTCGATCTTGCACATTATGAAGTTCCTGCTCCTGCGCC
TTCTGCAAACTCGTACCTTGTTTATGCGGCGGACAATGCAGAGAATCCAC
GATCTCTTTCAGCTTCTGGTTCATTTCGTAATGACAGTACTCCAAAAGCA
GCACAGAGAAATTCTGAAGATTCTGGTGTTACGGTTGATGGATCCCCATC
TGCAAAGGAAATGACGATCGTTGTTCCTGATTCCTCGAATATCTATTCGG
CTTTTGGAGAGGCTGAGTCAAAGTCTGTGGAGACACTCTCACCGTTTCAG
CAGAAAGATGGTCAAAAGGGACTCTTTGTTGATCGTGGTGTTGGCTCCCC
TAGGCTAGTGAAGTCTCTTGCTGCAAGCTCTTTTTTGATTGATACCAAGC
AAATCAAGAATTCAATGCCAGCCGCTGCAGGAGCTGTTGCCGCAGCAGCG
GTAGCTGATCAAATGCTTGGACCGAAAGAAGATAGACATCTAGCAATTGT
CCTGGTTGGCTTGCCTGCTCGAGGAAAGACTTTTACTGCAGCAAAGTTGA
CAAGATATCTACGCTGGTTGGGACATGATACTAAACATTTTAATGTTGGA
AAGTACCGACGGCTTAAACATGGAGTTAACATGAGTGCTGATTTCTTCCG
AGCTGATAATCCTGAAGGTGTAGAGGCACGGACTGAGGTAGCAGCACTTG
CTATGGAGGACATGATAGCTTGGATGCAAGAAGGTGGTCAAGTTGGAATC
TTTGATGCAACTAATAGCACACGAGTTCGACGGAATATGTTGATGAAAAT
GGCTGAAGGAAAGTGTAAGATCATTTTTCTGGAAACACTATGCAATGATG
AGCGCATTATTGAAAGAAACATACGTCTAAAAATCCAGCAAAGTCCTGAC
TATTCAGAAGAAATGGATTTTGAAGCTGGAGTACGAGACTTTAGAGACCG
TTTAGCCAATTATGAAAAGGTTTATGAGCCTGTAGAAGAAGGTTCGTACA
TCAAAATGATTGATATGGTCAGTGGGAACGGTGGACAGATACAAGTGAAC
AATATTAGTGGTTACCTTCCTGGGAGAATTGTATTTTTCTTGGTGAATAC
GCATCTCACCCCACGACCAATACTACTCACTCGGCATGGAGAAAGCATGG
ATAATGTTAGAGGCAGAATTGGAGGAGATAGCGTAATAAGTGACTCTGGT
AAACTTTACGCAAAGAAACTTGCCAGCTTTGTTGAAAAGCGACTTAAATC
TGAAAAAGCTGCTTCGATTTGGACCAGTACACTTCAGAGAACAAACCTAA
CAGCAAGTTCCATTGTCGGATTCCCCAAGGTGCAATGGCGTGCCCTGGAC
GAGATCAATGCTGGAGTTTGTGATGGGATGACATATGAAGAGGTAAAGAA
GAACATGCCAGAAGAATACGAATCTCGAAAAAAGGACAAATTACGATACC
GATACCCTCGTGGAGAGTCCTACCTTGATGTAATCCAACGGCTTGAACCT
GTGATCATTGAGTTAGAACGACAAAGAGCTCCTGTCGTCGTGATATCTCA
CCAGGCTGTTCTGAGAGCCTTGTATGCATATTTTGCGGATCGTCCCCTCA
AAGAAATACCCCAAATCGAGATGCCACTTCACACTATAATAGAGATACAG
ATGGGAGTTTCTGGTGTGCAAGAGAAACGCTACAAACTCATGGACTGAAT
TC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDGSGASKNTEEDDD
GSNGGGGQLYVSLKMENSKVEGELTPHVYGSLPLIGSWDPSKALPMQRES
ALMSELSFVVPPDHETLDFKFLLKPKNRNTPCIVEEGENRLLTGGSLQGD
ARLALFRLEGDVIVEFRVFINADRVSPIDLATSWRAYRENLQPSTVRGIP
DVSINPDPKSAECPLESLELDLAHYEVPAPAPSANSYLVYAADNAENPRS
LSASGSFRNDSTPKAAQRNSEDSGVTVDGSPSAKEMTIVVPDSSNIYSAF
GEAESKSVETLSPFQQKDGQKGLFVDRGVGSPRLVKSLAASSFLIDTKQI
KNSMPAAAGAVAAAAVADQMLGPKEDRHLAIVLVGLPARGKTFTAAKLTR
YLRWLGHDTKHFNVGKYRRLKHGVNMSADFFRADNPEGVEARTEVAALAM
EDMIAWMQEGGQVGIFDATNSTRVRRNMLMKMAEGKCKIIFLETLCNDER
IIERNIRLKIQQSPDYSEEMDFEAGVRDFRDRLANYEKVYEPVEEGSYIK
MIDMVSGNGGQIQVNNISGYLPGRIVFFLVNTHLTPRPILLTRHGESMDN
VRGRIGGDSVISDSGKLYAKKLASFVEKRLKSEKAASIWTSTLQRTNLTA
SSIVGFPKVQWRALDEINAGVCDGMTYEEVKKNMPEEYESRKKDKLRYRY
PRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKE
IPQIEMPLHTIIEIQMGVSGVQEKRYKLMD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 109900 Daltons