cDNA Clone CRAF

• Synonyms: CRAF, RAF1
• Plasmid: pGEX6P-1-d1-306 CRAF Y340D Y341D V492E
• Parent Plasmid: pGEX6P1
• Expressed: GST-d1-306 CRAF Y340D Y341D V492E
• Genbank: BC018119
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTCACAGCCGAAAACCCCCGTGCCAGCACAAAGAGAGCGGGCACC
AGTATCTGGGACCCAGGAGAAAAACAAAATTAGGCCTCGTGGACAGAGAG
ATTCAAGCGATGATTGGGAAATAGAAGCCAGTGAAGTGATGCTGTCCACT
CGGATTGGGTCAGGCTCTTTTGGAACTGTTTATAAGGGTAAATGGCACGG
AGATGTTGCAGTAAAGATCCTAAAGGTTGTCGACCCAACCCCAGAGCAAT
TCCAGGCCTTCAGGAATGAGGTGGCTGTTCTGCGCAAAACACGGCATGTG
AACATTCTGCTTTTCATGGGGTACATGACAAAGGACAACCTGGCAATTGT
GACCCAGTGGTGCGAGGGCAGCAGCCTCTACAAACACCTGCATGTCCAGG
AGACCAAGTTTCAGATGTTCCAGCTAATTGACATTGCCCGGCAGACGGCT
CAGGGAATGGACTATTTGCATGCAAAGAACATCATCCATAGAGACATGAA
ATCCAACAATATATTTCTCCATGAAGGCTTAACAGTGAAAATTGGAGATT
TTGGTTTGGCAACAGAAAAGTCACGCTGGAGTGGTTCTCAGCAGGTTGAA
CAACCTACTGGCTCTGTCCTCTGGATGGCCCCAGAGGTGATCCGAATGCA
GGATAACAACCCATTCAGTTTCCAGTCGGATGTCTACTCCTATGGCATCG
TATTGTATGAACTGATGACGGGGGAGCTTCCTTATTCTCACATCAACAAC
CGAGATCAGATCATCTTCATGGTGGGCCGAGGATATGCCTCCCCAGATCT
TAGTAAGCTATATAAGAACTGCCCCAAAGCAATGAAGAGGCTGGTAGCTG
ACTGTGTGAAGAAAGTAAAGGAAGAGAGGCCTCTTTTTCCCCAGATCCTG
TCTTCCATTGAGCTGCTCCAACACTCTCTACCGAAGATCAACCGGAGCGC
TTCCGAGCCATCCTTGCATCGGGCAGCCCACACTGAGGATATCAATGCTT
GCACGCTGACCACGTCCCCGAGGCTGCCTGTCTTCTAGGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSQPKTPVPAQRERAPVSGT
QEKNKIRPRGQRDSSDDWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAV
KILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWC
EGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNI
FLHEGLTVKIGDFGLATEKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNP
FSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLY
KNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPS
LHRAAHTEDINACTLTTSPRLPVF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU925
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag