cDNA Clone CRAF

• Synonyms: CRAF, RAF1
• Plasmid: pGEX6P-1-delta 1-306 CRAF Y340D Y341D
• Parent Plasmid: pGEX6P1
• Expressed: GST-delta 1-306 CRAF Y340D Y341D
• Genbank: BC018119
• Tag: GST
• Species: Human

Sequence of Insert

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSQPKTPVPAQRERAPVSGT
QEKNKIRPRGQRDSSDDWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAV
KILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWC
EGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNI
FLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNP
FSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLY
KNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPS
LHRAAHTEDINACTLTTSPRLPVF*

Amino Acid Sequence

GGATCCTCACAGCCGAAAACCCCCGTGCCAGCACAAAGAGAGCGGGCACC
AGTATCTGGGACCCAGGAGAAAAACAAAATTAGGCCTCGTGGACAGAGAG
ATTCAAGCGATGATTGGGAAATAGAAGCCAGTGAAGTGATGCTGTCCACT
CGGATTGGGTCAGGCTCTTTTGGAACTGTTTATAAGGGTAAATGGCACGG
AGATGTTGCAGTAAAGATCCTAAAGGTTGTCGACCCAACCCCAGAGCAAT
TCCAGGCCTTCAGGAATGAGGTGGCTGTTCTGCGCAAAACACGGCATGTG
AACATTCTGCTTTTCATGGGGTACATGACAAAGGACAACCTGGCAATTGT
GACCCAGTGGTGCGAGGGCAGCAGCCTCTACAAACACCTGCATGTCCAGG
AGACCAAGTTTCAGATGTTCCAGCTAATTGACATTGCCCGGCAGACGGCT
CAGGGAATGGACTATTTGCATGCAAAGAACATCATCCATAGAGACATGAA
ATCCAACAATATATTTCTCCATGAAGGCTTAACAGTGAAAATTGGAGATT
TTGGTTTGGCAACAGTAAAGTCACGCTGGAGTGGTTCTCAGCAGGTTGAA
CAACCTACTGGCTCTGTCCTCTGGATGGCCCCAGAGGTGATCCGAATGCA
GGATAACAACCCATTCAGTTTCCAGTCGGATGTCTACTCCTATGGCATCG
TATTGTATGAACTGATGACGGGGGAGCTTCCTTATTCTCACATCAACAAC
CGAGATCAGATCATCTTCATGGTGGGCCGAGGATATGCCTCCCCAGATCT
TAGTAAGCTATATAAGAACTGCCCCAAAGCAATGAAGAGGCTGGTAGCTG
ACTGTGTGAAGAAAGTAAAGGAAGAGAGGCCTCTTTTTCCCCAGATCCTG
TCTTCCATTGAGCTGCTCCAACACTCTCTACCGAAGATCAACCGGAGCGC
TTCCGAGCCATCCTTGCATCGGGCAGCCCACACTGAGGATATCAATGCTT
GCACGCTGACCACGTCCCCGAGGCTGCCTGTCTTCTAGGAATTC

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU810
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express N terminal GST fusion.