cDNA Clone MYPT1

• Plasmid: pMBP MYPT1
• Parent Plasmid: pMALP2X
• Expressed: MBP MYPT1
• Genbank: D37985
• Tag: MBP
• Species: Chicken

Sequence of Insert

GAATTCATGGCTTACCCATACGATGTTCCAGATTGCGCTAGCTTGGGTGG
TCATATGGCTAGAATTCGTTCCACACGAACCAGAGAACAAGAAAATGAAG
AAAAAGAAAAGGAAGAGAAAGAAAAGCAAGACAAAGAAAAACAAGAAGAA
AAGAAAGAATCTGAAACTAAAGATGATGATTATAGACAAAGATATTCCCG
AACTGTTGAAGAGCCATATCATCGCTATAGACCAACTTCAACTTCAACTT
CTACATCATCCACCTCTTCGCTCTCCACCTCCACTAGCTCTCTTTCAAGT
TCAAGTCAGCTAAACAGACCAAACAGCCTTATAGGTATAACTTCTGCCTA
TTCTCGGAGTGGAACAAAGGAAAGTGAGAGAGAAGGGGGCAAAAAAGAGG
AAGAGAAGGAGGAAGATAAGTCACAGCCCAAATCTATACGGGAAAGGCGG
CGACCAAGAGAAAAACGGCGATCTACAGGAGTTTCATTTTGGACTCAAGA
TAGTGATGAAAATGAACAGGAGCACCAGTCTGATTCAGAAGAAGGAACAA
ATAAGAAAGAAACTCAGAGTGATAGCCTTTCAAGATATGACACGGGATCA
CTCAGTGTGTCTTCGGGTGATCGTTACGATTCAGCACAGGGGCGATCTGG
ATCACAGAGTTACCTTGAAGATCGAAAACCTTACTGCAGTAGGCTAGAAA
AAGAAGATAGCACTGATTTTAAGAAGCTTTATGAACAGATCCTAGCTGAA
AACGAAAAGCTGAAAGCACAGTTACATGATACCAACATGGAACTTACAGA
TCTTAAACTACAGCTGGAGAAGACCACTCAGAGACAAGAAAGATTTGCTG
ACAGATCGCTCCTGGAAATGGAGAAAAGGGTGTCCGGCAAGAGTCAGTAT
CTACTGGGCGGAAAGAAGAGCTCTAGAAAGAAGGATATCTGA

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFMAYPYDVPDCASLGGHMARIRSTRTREQENEEKE
KEEKEKQDKEKQEEKKESETKDDDYRQRYSRTVEEPYHRYRPTSTSTSTS
STSSLSTSTSSLSSSSQLNRPNSLIGITSAYSRSGTKESEREGGKKEEEK
EEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEHQSDSEEGTNKK
ETQSDSLSRYDTGSLSVSSGDRYDSAQGRSGSQSYLEDRKPYCSRLEKED
STDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKTTQRQERFADRS
LLEMEKRVSGKSQYLLGGKKSSRKKDI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU652
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal MBP fusion for bacterial expression.