cDNA Clone NEK6

• Plasmid: BAC-NEK6
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Nek6
• Genbank: NM_014397
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCAAAGGCCTACGTCGACGAGCTCACTAGTGCCACCATGGACTACA
AGGACGACGATGACAAGGCAGGACAGCCCGGCCACATGCCCCATGGAGGG
AGTTCCAACAACCTCTGCCACACCCTGGGGCCTGTGCATCCTCCTGACCC
ACAGAGGCATCCCAACACGCTGTCTTTTCGCTGCTCGCTGGCGGACTTCC
AGATCGAAAAGAAGATAGGCCGAGGACAGTTCAGCGAGGTGTACAAGGCC
ACCTGCCTGCTGGACAGGAAGACAGTGGCTCTGAAGAAGGTGCAGATCTT
TGAGATGATGGACGCCAAGGCGAGGCAGGACTGTGTCAAGGAGATCGGCC
TCTTGAAGCAACTGAACCACCCAAATATCATCAAGTATTTGGACTCGTTT
ATCGAAGACAACGAGCTGAACATTGTGCTGGAGTTGGCTGACGCAGGGGA
CCTCTCGCAGATGATCAAGTACTTTAAGAAGCAGAAGCGGCTCATCCCGG
AGAGGACAGTATGGAAGTACTTTGTGCAGCTGTGCAGCGCCGTGGAGCAC
ATGCATTCACGCCGGGTGATGCACCGAGACATCAAGCCTGCCAACGTGTT
CATCACAGCCACGGGCGTCGTGAAGCTCGGTGACCTTGGTCTGGGCCGCT
TCTTCAGCTCTGAGACCACCGCAGCCCACTCCCTAGTGGGGACGCCCTAC
TACATGTCACCGGAGAGGATCCATGAGAACGGCTACAACTTCAAGTCCGA
CATCTGGTCCTTGGGCTGTCTGCTGTACGAGATGGCAGCCCTCCAGAGCC
CCTTCTATGGAGATAAGATGAATCTCTTCTCCCTGTGCCAGAAGATCGAG
CAGTGTGACTACCCCCCACTCCCCGGGGAGCACTACTCCGAGAAGTTACG
AGAACTGGTCAGCATGTGCATCTGCCCTGACCCCCACCAGAGACCTGACA
TCGGATACGTGCACCAGGTGGCCAAGCAGATGCACATCTGGATGTCCAGC
ACCTGAACTAGTCGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDELTSATMDYKDDDD
KAGQPGHMPHGGSSNNLCHTLGPVHPPDPQRHPNTLSFRCSLADFQIEKK
IGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQL
NHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVW
KYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSE
TTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGD
KMNLFSLCQKIEQCDYPPLPGEHYSEKLRELVSMCICPDPHQRPDIGYVH
QVAKQMHIWMSST*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU635
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His with FLAG tag