cDNA Clone BRAF

• Synonyms: RAF-b isoform
• Plasmid: pFBHTc BRAF V599E
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-BRAF V599E
• Genbank: NP_004324.2
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACGCGGCGCTGAGCGGTGGCGGTGGTGGCGGCGCGGAGCCGGGCCA
GGCTCTGTTCAACGGGGACATGGAGCCCGAGGCCGGCGCCGGCGCCGGCG
CCGCGGCCTCTTCGGCTGCGGACCCTGCCATTCCGGAGGAGGTGTGGAAT
ATCAAACAAATGATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGA
CAAATTTGGTGGGGAGCATAATCCACCATCAATATATCTGGAGGCCTATG
AAGAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTA
TTGGAATCTCTGGGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATC
AATGGATACCGTTACATCTTCTTCCTCTTCTAGCCTTTCAGTGCTACCTT
CATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCAACCCC
AAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG
GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGA
AAGCACTGATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGA
ATTCAGGATGGAGAGAAGAAACCAATTGGTTGGGACACTGATATTTCCTG
GCTTACTGGAGAAAAATTGCATGTGGAAGTGTTGGGGAATGTTCCACTTA
CAACACACAACTTTGTACGAAAAACGTTTTTCACCTTAGCATTTTGTGAC
TTTTGTCGAAAGCTGCTTTTCCAGGGTTTCCGCTGTCAAACATGTGGTTA
TAAATTTCACCAGCGTTGTAGTACAGAAGTTCCACTGATGTGTGTTAATT
ATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAACACCACCCA
ATACCACAGGAAGAGGCGTCCTTAGCAGAGACTGCCCTAACATCTGGATC
ATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTCACCA
GTCCGTCTCCTTCAAAATCCATTCCAATTCCACAGCCCTTCCGACCAGCA
GATGAAGATCATCGAAATCAATTTGGGCAACGAGACCGATCCTCATCAGC
TCCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGA
TTAGAGACCAAGGATTTCGTGGTGATGGAGGATCAACCACAGGTTTGTCT
GCTACCCCCCCTGCCTCATTACCTGGCTCACTAACTAACGTGAAAGCCTT
ACAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAG
AAGACAGGAATCGAATGAAAACACTTGGTAGACGGGACTCGAGTGATGAT
TGGGAGATTCCTGATGGGCAGATTACAGTGGGACAAAGAATTGGATCTGG
ATCATTTGGAACAGTCTACAAGGGAAAGTGGCATGGTGATGTGGCAGTGA
AAATGTTGAATGTGACAGCACCTACACCTCAGCAGTTACAAGCCTTCAAA
AATGAAGTAGGAGTACTCAGGAAAACACGACATGTGAATATCCTACTCTT
CATGGGCTATTCCACAAAGCCACAACTGGCTATTGTTACCCAGTGGTGTG
AGGGCTCCAGCTTGTATCACCATCTCCATATCATTGAGACCAAATTTGAG
ATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATGGATTA
CTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATAT
TTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACA
GAAAAATCTCGATGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATC
CATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCAT
ACAGCTTTCAGTCAGATGTATATGCATTTGGGATTGTTCTGTATGAATTG
ATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAGATAAT
TTTTATGGTGGGACGAGGATACCTGTCTCCAGATCTCAGTAAGGTACGGA
GTAACTGTCCAAAAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAG
AAAAGAGATGAGAGACCACTCTTTCCCCAAATTCTCGCCTCTATTGAGCT
GCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCCTCCT
TGAATCGGGCTGGTTTCCAAACAGAGGATTTTAGTCTATATGCTTGTGCT
TCTCCAAAAACACCCATCCAGGCAGGGGGATATGGTGCGTTTCCTGTCCA
CTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDAALSGGGGGGAEPG
QALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALL
DKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSA
SMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQ
RTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDIS
WLTGEKLHVEVLGNVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCG
YKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSG
SSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHPNQFGQRDRSSS
APNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKA
LQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGS
GSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILL
FMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMD
YLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATEKSRWSGSHQFEQLSG
SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQI
IFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIE
LLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPV
H*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU630
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His C terminal fusion. As Genbank except E256K E263G