cDNA Clone BRAF

• Synonyms: RAF-b isoform
• Plasmid: pFBHTc delta N 1-416 BRAF V599E
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-delta N 1-416 BRAF V599E
• Genbank: XM_035107
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACCAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATC
CTCAGAAGACAGGAATCGAATGAAAACACTTGGTAGACGGGACTCGAGTG
ATGATTGGGAGATTCCTGATGGGCAGATTACAGTGGGACAAAGAATTGGA
TCTGGATCATTTGGAACAGTCTACAAGGGAAAGTGGCATGGTGATGTGGC
AGTGAAAATGTTGAATGTGACAGCACCTACACCTCAGCAGTTACAAGCCT
TCAAAAATGAAGTAGGAGTACTCAGGAAAACACGACATGTGAATATCCTA
CTCTTCATGGGCTATTCCACAAAGCCACAACTGGCTATTGTTACCCAGTG
GTGTGAGGGCTCCAGCTTGTATCACCATCTCCATATCATTGAGACCAAAT
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG
GATTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAA
TATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAG
CTACAGTGAAATCTCGATGGAGTGGGTCCCATCAGTTTGAACAGTTGTCT
GGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAA
TCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATTGTTCTGTATG
AATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG
ATAATTTTTATGGTGGGACGAGGATACCTGTCTCCAGATCTCAGTAAGGT
ACGGAGTAACTGTCCAAAAGCCATGAAGAGATTAATGGCAGAGTGCCTCA
AAAAGAAAAGAGATGAGAGACCACTCTTTCCCCAAATTCTCGCCTCTATT
GAGCTGCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACC
CTCCTTGAATCGGGCTGGTTTCCAAACAGAGGATTTTAGTCTATATGCTT
GTGCTTCTCCAAAAACACCCATCCAGGCAGGGGGATATGGTGCGTTTCCT
GTCCACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDQKSPGPQRERKSSS
SSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV
AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQ
WCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSN
NIFLHEDLTVKIGDFGLATEKSRWSGSHQFEQLSGSILWMAPEVIRMQDK
NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK
VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASE
PSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU603
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His C terminal fusion.