cDNA Clone CRAF

• Synonyms: RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
• Plasmid: pFastbac Htb CRAF iso b
• Parent Plasmid: pFastBac HTb
• Expressed: 6His TEV CRAF iso b
• Genbank: NM_002880.4
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccgagcacatacagggagcttggaagacgatcagcaatggttttgg
attcaaagatgccgtgtttgatggctccagctgcatctctcctacaatag
ttcagcagtttggctatcagcgccgggcatcagatgatggcaaactcaca
gatccttctaagacaagcaacactatccgtgttttcttgccgaacaagca
aagaacagtggtcaatgtgcgaaatggaatgagcttgcatgactgcctta
tgaaagcactcaaggtgaggggcctgcaaccagagtgctgtgcagtgttc
agacttctccacgaacacaaaggtaaaaaagcacgcttagattggaatac
tgatgctgcgtctttgattggagaagaacttcaagtagatttcctggatc
atgttcccctcacaacacacaactttgctcggaagacgttcctgaagctt
gccttctgtgacatctgtcagaaattcctgctcaatggatttcgatgtca
gacttgtggctacaaatttcatgagcactgtagcaccaaagtacctacta
tgtgtgtggactggagtaacatcagacaactcttattgtttccaaattcc
actattggtgatagtggagtcccagcactaccttctttgactatgcgtcg
tatgcgagagtctgtttccaggatgcctgttagttctcagcacagatatt
ctacacctcacgccttcacctttaacacctccagtccctcatctgaaggt
tccctctcccagaggcagaggtcgacatccacacctaatgtccacatggt
cagcaccaccctgcctgtggacagcaggatgattgaggatgcaattcgaa
gtcacagcgaatcagcctcaccttcagccctgtccagtagccccaacaat
ctgagcccaacaggctggtcacagccgaaaacccccgtgccagcacaaag
agagcgggcaccagtatctgggacccaggagaaaaacaaaattaggcctc
gtggacagagagattcaagctattattgggaaatagaagccagtgaagtg
atgctgtccactcggattgggtcaggctcttttggaactgtttataaggg
taaatggcacggagatgttgcagtaaagatcctaaaggttgtcgacccaa
ccccagagcaattccaggccttcaggaatgaggtggctgttctgcgcaaa
acacggcatgtgaacattctgcttttcatggggtacatgacaaaggacaa
cctggcaattgtgacccagtggtgcgagggcagcagcctctacaaacacc
tgcatgtccaggagaccaagtttcagatgttccagctaattgacattgcc
cggcagacggctcagggaatggactatttgcatgcaaagaacatcatcca
tagagacatgaaatccaacaatatatttctccatgaaggcttaacagtga
aaattggagattttggtttggcaacagtaaagtcacgctggagtggttct
cagcaggttgaacaacctactggctctgtcctctggatggccccagaggt
gatccgaatgcaggataacaacccattcagtttccagtcggatgtctact
cctatggcatcgtattgtatgaactgatgacgggggagcttccttattct
cacatcaacaaccgagatcagatcatcttcatggtgggccgaggatatgc
ctccccagatcttagtaagctatataagaactgccccaaagcaatgaaga
ggctggtagctgactgtgtgaagaaagtaaaggaagagaggcctcttttt
ccccagatcctgtcttccattgagctgctccaacactctctaccgaagat
caaccggagcgcttccgagccatccttgcatcgggcagcccacactgagg
atatcaatgcttgcacgctgaccacgtccccgaggctgcctgtcttctag
gaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSEHIQGAWKTISNGFGFKDAVFD
GSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVR
NGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIG
EELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFH
EHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSR
MPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVD
SRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSG
TQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVA
VKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQW
CEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNN
IFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNN
PFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKL
YKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEP
SLHRAAHTEDINACTLTTSPRLPVF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbac Htb CRAF iso b91.04 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU572
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion. Isoform b - variant 2
• Low Temp: No