cDNA Clone BMH1 (yeast)

• Plasmid: pTRC-His BMH1
• Parent Plasmid: pTRC
• Genbank: CAA46959
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGTCAACCAGTCGTGAAGATTCTGTGTACCTAGCCAAGTTGGC
TGAACAGGCCGAACGTTATGAAGAAATGGTCGAAAACATGAAGACTGTTG
CCTCCTCTGGCCAAGAGTTGTCGGTCGAAGAGCGTAATTTGTTGTCTGTT
GCTTATAAGAACGTTATTGGTGCTCGTCGTGCCTCTTGGAGAATTGTTTC
TTCTATTGAGCAAAAGGAGGAGTCCAAGGAGAAGTCCGAACACCAGGTCG
AGTTGATTTGTTCGTACCGTTCGAAGATTGAGACCGAACTAACTAAGATC
TCCGACGATATTTTGTCCGTGCTAGACTCCCACTTAATTCCATCAGCCAC
CACTGGCGAGTCCAAGGTTTTCTACTATAAGATGAAGGGTGACTACCACC
GTTATTTGGCTGAATTTTCTAGTGGCGATGCTAGAGAAAAGGCCACAAAC
GCCTCTTTAGAAGCATACAAGACCGCTTCTGAAATTGCCACCACAGAGTT
ACCCCCAACTCACCCAATCCGTCTAGGTTTGGCTCTTAACTTCTCTGTCT
TCTATTATGAAATTCAAAACTCTCCAGACAAAGCCTGCCATTTGCGCAAG
CAAGCTTTTGACGACGCTATTGCTGAGTTGGACACTCTGTCTGAAGAATC
ATACAAAGATAGCACACTTATCATGCAACTGCTAAGGGACAATTTAACCT
TATGGACTTCAGACATGTCCGAGTCCGGTCAAGCTGAAGACCAACAACAA
CAACAACAACATCAGCAACAGCAGCCACCTGCTGCCGCCGAAGGTGAAGC
ACCAAAGTAAGAATTC

Amino Acid Sequence

MGGSHHHHHHGMASMTGGQQMGRTLYDDDDKDRWGSMSTSREDSVYLAKL
AEQAERYEEMVENMKTVASSGQELSVEERNLLSVAYKNVIGARRASWRIV
SSIEQKEESKEKSEHQVELICSYRSKIETELTKISDDILSVLDSHLIPSA
TTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNASLEAYKTASEIATTE
LPPTHPIRLGLALNFSVFYYEIQNSPDKACHLRKQAFDDAIAELDTLSEE
SYKDSTLIMQLLRDNLTLWTSDMSESGQAEDQQQQQQHQQQQPPAAAEGE
APK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU383
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression; Calc Mol Weight 34191 Da