cDNA Clone MMS22L

• Plasmid: pcDNA5-FRT/TO-FLAG MMS22L de novo
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Expressed: FLAG-MMS22L de novo
• Genbank: NM_198468.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCAATGGAGAACTGTTCTGCTGCATCGACGTTCCTGACTGACAGCTTAGAGCTGGAGCTGGGGACGGAATGGTGCAAACCTCCTTACTTTTCTTGTGCTGTTGACAACAGAGGAGGAGGAAAACATTTTTCTGGAGAATCCTACCTCTGCAGCGGAGCCCTTAAGCGATTGATTTTGAATCTTGACCCTCTACCAACTAATTTTGAAGAAGATACCTTGGAAATATTTGGCATTCAGTGGGTTACTGAAACAGCATTAGTGAATTCATCTAGAGAACTCTTTCATTTATTCAGGCAACAACTGTACAACTTGGAAACCTTGTTACAGTCCAGTTGTGATTTTGGGAAGGTATCAACTCTACACTGCAAAGCAGACAATATTAGGCAGCAGTGTGTACTATTTCTCCATTATGTTAAAGTTTTCATCTTCAGGTATCTGAAAGTACAGAATGCTGAGAGTCATGTTCCTGTCCATCCTTATGAGGCTTTGGAGGCTCAGCTTCCCTCAGTGTTGATTGATGAGCTTCATGGATTACTCTTGTATATTGGACACCTATCTGAACTTCCCAGTGTTAATATAGGAGCATTTGTAAATCAAAACCAGATTAAGCTTTTTCCACCGTCATGGCATTTATTACATCTCCACTTGGATATACATTGGCTGGTGCTAGAAATTCTTTACATGCTGGGTGAAAAATTGAAACAAGTTGTATATGGTCATCAGTTTATGAATCTGGCAAGTGACAATTTAACCAACATCAGCCTATTTGAAGAACATTGTGAAACTCTCCTTTGTGATTTAATAAGCCTGTCACTCAACAGGTACGACAAGGTTAGGTCTTCTGAATCATTAATGAGTGACCAGTGTCCATGTTTATGCATTAAAGAATTATGGGTTCTACTTATTCATCTTCTAGACCACAGAAGTAAATGGTTTGTCTCGGAATCATTTTGGAACTGGTTGAATAAACTACTTAAAACACTGCTTGAAAAATCAAGTGACCGAAGAAGATCCTCTATGCCTGTAATCCAGTCCAGGGATCCATTAGGTTTTAGTTGGTGGATTATTACTCATGTAGCATCATTTTACAAGTTTGATCGCCATGGAGTACCAGATGAAATGAGAAAAGTGGAATCAAATTGGAACTTTGTAGAAGAACTGCTGAAAAAGTCCATCAGTGTTCAGGGTGTCATTCTAGAAGAACAATTACGAATGTATCTTCACTGTTGTTTGACACTTTGTGATTTCTGGGAGCCAAACATTGCAATTGTTACCATTTTATGGGAATATTATAGTAAGAACCTGAATAGTTCCTTCAGTATTTCTTGGCTTCCTTTTAAAGGCCTTGCTAATACCATGAAGTCACCCTTGTCTATGCTTGAAATGGTGAAGACTTGCTGTTGCGATAAACAAGATCAGGAACTATATAAATCCAGCAGTAGTTATACTATTTTTCTTTGTATTCTGGCAAAAGTTGTTAAAAAAGCAATGAAGAGCAATGGCCCTCATCCTTGGAAACAAGTCAAAGGAAGAATATATTCAAAATTCCATCAAAAAAGAATGGAAGAACTAACTGAAGTTGGTCTACAGAACTTTTTTAGCCTTTTTCTACTGTTAGCAGCTGTTGCAGAGGTAGAAGATGTTGCAAGTCATGTTTTAGACCTCCTGAATTTCCTCAAGCCTGCTTTTGTAACGTCTCAGAGAGCCCTCATTTGGAAGGGTCACATGGCCTTCCTCTTGATGTATGCCCAGAAAAATCTGGACATTGGTGTTTTGGCTGAGAAATTTTCATGTGCTTTCCGGGAGAAAGCAAAGGAATTCTTGGTGTCTAAGAATGAGGAAATGGTACAGAGACAGACTATCTGGACCCTTCTTTCCATATACATTGATGGTGTTCAAGAAGTGTTTGAGACCAGCTATTGCTTGTATCCTTCCCATGAAAAACTGCTTAATGATGGGTTTAGTATGCTTCTGCGAGCATGTCGAGAATCTGAACTTAGGACAGTATTGAGCTTCCTACAAGCTGTTCTGGCCAGAATCAGGAGTATGCATCAACAATTGTGTCAGGAACTTCAAAGGGACAATGTGGACCTATTTGTACAGTCTTCATTATCGGCTAAAGAGCGCCACCTTGCTGCAGTTGCCAGTGCACTGTGGAGACATTTCTTTTCATTTTTGAAGAGTCAGAGAATGTCACAGGTAGTGCCTTTCTCACAACTTGCGGATGCAGCTGCAGACTTTACTTTGCTAGCAATGGACATGCCAAGCACAGCTCCATCAGATTTTCAGCCTCAGCCAGTTATATCAATTATTCAACTTTTTGGTTGGGATGATATCATCTGCCCTCAAGTTGTAGCAAGATATTTAAGTCATGTCCTACAAAATAGCACATTATGTGAAGCACTTTCTCATTCAGGCTATGTATCTTTTCAAGCCTTAACCGTAAGATCATGGATTCGTTGTGTTTTGCAAATGTATATTAAAAACCTCTCTGGGCCTGATGATTTGCTCATAGATAAAAATCTGGAAGAGGCAGTTGAAAAAGAGTACATGAAACAGTTGGTCAAACTGACAAGATTACTATTTAATCTCTCAGAAGTAAAGAGTATTTTCTCAAAGGCCCAAGTTGAATATTTATCCATCTCAGAAGACCCTAAAAAAGCACTTGTTCGATTCTTTGAGGCTGTTGGTGTAACTTACGGGAACGTCCAGACACTTTCTGATAAATCTGCCATGGTCACAAAGTCCTTGGAATACCTTGGTGAAGTATTAAAATATATTAAGCCTTATTTGGGAAAAAAAGTTTTCAGTGCAGGGCTGCAGCTGACTTATGGAATGATGGGAATTCTTGTGAAGTCATGGGCACAAATCTTTGCCACTTCTAAAGCCCAAAAATTACTATTCCGGATCATAGATTGTTTACTGCTGCCACATGCAGTATTACAGCAAGAGAAGGAACTGCCTGCACCTATGTTGTCAGCAATTCAGAAAAGTCTTCCTTTGTATCTCCAGGGCATGTGTATCGTGTGTTGTCAATCTCAAAATCCGAATGCCTATTTGAATCAGTTGCTAGGGAATGTTATTGAGCAGTATATTGGGCGATTTCTTCCAGCTTCACCATATGTTTCAGATCTTGGACAACATCCTGTTTTGCTGGCATTGAGAAACACAGCCACTATTCCACCAATATCATCTCTAAAGAAATGCATTGTGCAAGTCATAAGGAAATCCTACCTTGAGTATAAGGGGTCCTCACCTCCTCCTCGCTTAGCATCCATTCTGGCCTTCATCCTCCAACTCTTCAAGGAAACTAACACAGACATTTATGAAGTTGAACTACTCCTCCCTGGCATTTTAAAATGCTTGGTGTTAGTCAGTGAACCACAAGTTAAAAGGCTGGCCACAGAGAACCTGCAATACATGGTAAAAGCCTGCCAAGTGGGGTCAGAAGAAGAACCTTCCTCCCAGCTGACTTCTGTGTTTAGGCAGTTTATCCAGGATTATGGTATGAGGTACTATTACCAGGTTTACAGCATTTTAGAAACAGTAGCAACATTGGACCAGCAGGTTGTCATCCACTTGATTTCTACCCTTACTCAGTCTCTGAAGGATTCAGAGCAGAAATGGGGCCTTGGCAGGAATATAGCACAAAGGGAAGCCTATAGCAAACTTTTGTCTCACCTTGGACAGATGGGACAAGATGAGATGCAGAGACTGGAAAATGATAATACTTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSGESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYNLETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYEALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIHWLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDKVRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDRRRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSISVQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLANTMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVKGRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQRALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLLSIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSMHQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLADAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLCEALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKLTRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSDKSAMVTKSLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDCLLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQYIGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPRLASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQVGSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDSEQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT*

• Vector Type: Mammalian
• DU Number: DU37176
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression of protein of interest with N-ter FLAG tag.; ; Estimated MW of expressed protein : 144596.45 Daltons