cDNA Clone MMS22L

• Plasmid: pcDNA5-FRT/TO-GFP MMS22L de novo
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-MMS22L
• Genbank: NM_198468.2
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCAATGGAGAACTGTTCTGCTGCATCGACGTTCCTGACTGACAGCTTAGAGCTGGAGCTGGGGACGGAATGGTGCAAACCTCCTTACTTTTCTTGTGCTGTTGACAACAGAGGAGGAGGAAAACATTTTTCTGGAGAATCCTACCTCTGCAGCGGAGCCCTTAAGCGATTGATTTTGAATCTTGACCCTCTACCAACTAATTTTGAAGAAGATACCTTGGAAATATTTGGCATTCAGTGGGTTACTGAAACAGCATTAGTGAATTCATCTAGAGAACTCTTTCATTTATTCAGGCAACAACTGTACAACTTGGAAACCTTGTTACAGTCCAGTTGTGATTTTGGGAAGGTATCAACTCTACACTGCAAAGCAGACAATATTAGGCAGCAGTGTGTACTATTTCTCCATTATGTTAAAGTTTTCATCTTCAGGTATCTGAAAGTACAGAATGCTGAGAGTCATGTTCCTGTCCATCCTTATGAGGCTTTGGAGGCTCAGCTTCCCTCAGTGTTGATTGATGAGCTTCATGGATTACTCTTGTATATTGGACACCTATCTGAACTTCCCAGTGTTAATATAGGAGCATTTGTAAATCAAAACCAGATTAAGCTTTTTCCACCGTCATGGCATTTATTACATCTCCACTTGGATATACATTGGCTGGTGCTAGAAATTCTTTACATGCTGGGTGAAAAATTGAAACAAGTTGTATATGGTCATCAGTTTATGAATCTGGCAAGTGACAATTTAACCAACATCAGCCTATTTGAAGAACATTGTGAAACTCTCCTTTGTGATTTAATAAGCCTGTCACTCAACAGGTACGACAAGGTTAGGTCTTCTGAATCATTAATGAGTGACCAGTGTCCATGTTTATGCATTAAAGAATTATGGGTTCTACTTATTCATCTTCTAGACCACAGAAGTAAATGGTTTGTCTCGGAATCATTTTGGAACTGGTTGAATAAACTACTTAAAACACTGCTTGAAAAATCAAGTGACCGAAGAAGATCCTCTATGCCTGTAATCCAGTCCAGGGATCCATTAGGTTTTAGTTGGTGGATTATTACTCATGTAGCATCATTTTACAAGTTTGATCGCCATGGAGTACCAGATGAAATGAGAAAAGTGGAATCAAATTGGAACTTTGTAGAAGAACTGCTGAAAAAGTCCATCAGTGTTCAGGGTGTCATTCTAGAAGAACAATTACGAATGTATCTTCACTGTTGTTTGACACTTTGTGATTTCTGGGAGCCAAACATTGCAATTGTTACCATTTTATGGGAATATTATAGTAAGAACCTGAATAGTTCCTTCAGTATTTCTTGGCTTCCTTTTAAAGGCCTTGCTAATACCATGAAGTCACCCTTGTCTATGCTTGAAATGGTGAAGACTTGCTGTTGCGATAAACAAGATCAGGAACTATATAAATCCAGCAGTAGTTATACTATTTTTCTTTGTATTCTGGCAAAAGTTGTTAAAAAAGCAATGAAGAGCAATGGCCCTCATCCTTGGAAACAAGTCAAAGGAAGAATATATTCAAAATTCCATCAAAAAAGAATGGAAGAACTAACTGAAGTTGGTCTACAGAACTTTTTTAGCCTTTTTCTACTGTTAGCAGCTGTTGCAGAGGTAGAAGATGTTGCAAGTCATGTTTTAGACCTCCTGAATTTCCTCAAGCCTGCTTTTGTAACGTCTCAGAGAGCCCTCATTTGGAAGGGTCACATGGCCTTCCTCTTGATGTATGCCCAGAAAAATCTGGACATTGGTGTTTTGGCTGAGAAATTTTCATGTGCTTTCCGGGAGAAAGCAAAGGAATTCTTGGTGTCTAAGAATGAGGAAATGGTACAGAGACAGACTATCTGGACCCTTCTTTCCATATACATTGATGGTGTTCAAGAAGTGTTTGAGACCAGCTATTGCTTGTATCCTTCCCATGAAAAACTGCTTAATGATGGGTTTAGTATGCTTCTGCGAGCATGTCGAGAATCTGAACTTAGGACAGTATTGAGCTTCCTACAAGCTGTTCTGGCCAGAATCAGGAGTATGCATCAACAATTGTGTCAGGAACTTCAAAGGGACAATGTGGACCTATTTGTACAGTCTTCATTATCGGCTAAAGAGCGCCACCTTGCTGCAGTTGCCAGTGCACTGTGGAGACATTTCTTTTCATTTTTGAAGAGTCAGAGAATGTCACAGGTAGTGCCTTTCTCACAACTTGCGGATGCAGCTGCAGACTTTACTTTGCTAGCAATGGACATGCCAAGCACAGCTCCATCAGATTTTCAGCCTCAGCCAGTTATATCAATTATTCAACTTTTTGGTTGGGATGATATCATCTGCCCTCAAGTTGTAGCAAGATATTTAAGTCATGTCCTACAAAATAGCACATTATGTGAAGCACTTTCTCATTCAGGCTATGTATCTTTTCAAGCCTTAACCGTAAGATCATGGATTCGTTGTGTTTTGCAAATGTATATTAAAAACCTCTCTGGGCCTGATGATTTGCTCATAGATAAAAATCTGGAAGAGGCAGTTGAAAAAGAGTACATGAAACAGTTGGTCAAACTGACAAGATTACTATTTAATCTCTCAGAAGTAAAGAGTATTTTCTCAAAGGCCCAAGTTGAATATTTATCCATCTCAGAAGACCCTAAAAAAGCACTTGTTCGATTCTTTGAGGCTGTTGGTGTAACTTACGGGAACGTCCAGACACTTTCTGATAAATCTGCCATGGTCACAAAGTCCTTGGAATACCTTGGTGAAGTATTAAAATATATTAAGCCTTATTTGGGAAAAAAAGTTTTCAGTGCAGGGCTGCAGCTGACTTATGGAATGATGGGAATTCTTGTGAAGTCATGGGCACAAATCTTTGCCACTTCTAAAGCCCAAAAATTACTATTCCGGATCATAGATTGTTTACTGCTGCCACATGCAGTATTACAGCAAGAGAAGGAACTGCCTGCACCTATGTTGTCAGCAATTCAGAAAAGTCTTCCTTTGTATCTCCAGGGCATGTGTATCGTGTGTTGTCAATCTCAAAATCCGAATGCCTATTTGAATCAGTTGCTAGGGAATGTTATTGAGCAGTATATTGGGCGATTTCTTCCAGCTTCACCATATGTTTCAGATCTTGGACAACATCCTGTTTTGCTGGCATTGAGAAACACAGCCACTATTCCACCAATATCATCTCTAAAGAAATGCATTGTGCAAGTCATAAGGAAATCCTACCTTGAGTATAAGGGGTCCTCACCTCCTCCTCGCTTAGCATCCATTCTGGCCTTCATCCTCCAACTCTTCAAGGAAACTAACACAGACATTTATGAAGTTGAACTACTCCTCCCTGGCATTTTAAAATGCTTGGTGTTAGTCAGTGAACCACAAGTTAAAAGGCTGGCCACAGAGAACCTGCAATACATGGTAAAAGCCTGCCAAGTGGGGTCAGAAGAAGAACCTTCCTCCCAGCTGACTTCTGTGTTTAGGCAGTTTATCCAGGATTATGGTATGAGGTACTATTACCAGGTTTACAGCATTTTAGAAACAGTAGCAACATTGGACCAGCAGGTTGTCATCCACTTGATTTCTACCCTTACTCAGTCTCTGAAGGATTCAGAGCAGAAATGGGGCCTTGGCAGGAATATAGCACAAAGGGAAGCCTATAGCAAACTTTTGTCTCACCTTGGACAGATGGGACAAGATGAGATGCAGAGACTGGAAAATGATAATACTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTIDAAAMENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSGESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYNLETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYEALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIHWLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDKVRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDRRRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSISVQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLANTMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVKGRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQRALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLLSIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSMHQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLADAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLCEALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKLTRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVG
VTYGNVQTLSDKSAMVTKSLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDCLLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQYIGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPRLASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQVGSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDSEQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU37130
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression of protein of interest with N-ter GFP fusion.; ; Estimated MW of expressed protein : 170617.00 Daltons