cDNA Clone MYSM1

• Synonyms: 2A-DUB, 2ADUB
• Plasmid: pET15b MYSM1 S546L
• Parent Plasmid: pET15b original
• Expressed: 6xHis-MYSM1 S546L
• Genbank: NM_001085487.2
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGGCGGCTGAAGAGGCGGATGTGGATATCGAAGGGGACGTGGTAGCGGCGGCGGGGGCACAGCCAGGAAGTGGTGAAAATACAGCATCAGTTTTACAAAAAGATCACTATCTTGATTCATCTTGGAGAACAGAGAATGGCCTTATTCCTTGGACCTTGGATAACACCATCAGTGAAGAGAACAGAGCTGTTATTGAGAAAATGTTGTTGGAAGAAGAATATTATTTATCTAAAAAATCACAACCGGAAAAAGTCTGGCTTGATCAAAAGGAAGATGATAAAAAATACATGAAGAGTCTGCAGAAAACAGCAAAAATCATGGTACACTCTCCTACAAAACCAGCCAGTTACTCAGTAAAGTGGACGATAGAAGAAAAAGAGCTGTTTGAACAAGGGCTGGCTAAATTTGGCCGAAGATGGACCAAAATTTCAAAGCTAATTGGAAGCCGCACTGTTTTACAAGTGAAGAGTTATGCAAGACAGTATTTTAAAAATAAGGTCAAATGCGGTCTGGATAAAGAAACACCAAATCAGAAGACCGGCCATAATCTTCAAGTTAAAAATGAAGATAAAGGGACAAAGGCATGGACACCGTCATGTTTAAGGGGACGTGCTGATCCCAACTTGAATGCTGTAAAAATTGAAAAGTTATCTGATGATGAAGAAGTAGACATCACAGATGAGGTGGACGAGTTGTCTTCTCAAACACCCCAGAAGAATTCTAGCAGTGATCTTTTGTTAGACTTTCCTAATAGTAAAATGCATGAAACCAATCAAGGAGAATTCATTGCTTCTGACAGCCAGGAAGCTCTCTTTTCTAAGTCTTCCAGGGGCTGTCTTCAAAATGAAAAGCAAGATGAAACACTTTCAAGCTCAGAAATTACACTGTGGACTGAGAAACAGAGCAATGGTGACAAAAAATCAATTGAATTAAATGACCAGAAATTTAATGAATTGATTAAAAACTGCAACAAGCATGATGGAAGGGGAATAATAGTTGATGCCAGGCAGTTGCCTTCTCCAGAGCCTTGTGAAATTCAGAAAAATTTGAATGATAATGAAATGCTTTTTCATTCTTGCCAAATGGTAGAGGAAAGCCATGAGGAAGAAGAGCTTAAGCCACCAGAACAGGAAATAGAAATAGATAGAAATATCATTCAAGAAGAAGAAAAACAAGCAATTCCTGAGTTTTTTGAGGGGCGCCAAGCTAAAACACCAGAACGCTATTTGAAAATTAGAAATTATATTTTGGATCAATGGGAGATATGCAAACCGAAATACTTAAATAAGACCTCAGTACGTCCTGGCCTGAAGAACTGTGGAGATGTTAATTGTATTGGACGGATTCATACATACCTCGAATTGATAGGAGCAATCAATTTTGGATGTGAACAGGCTGTGTATAATAGGCCACAAACAGTTGACAAAGTACGAATCAGAGACAGAAAAGATGCAGTAGAAGCATACCAACTTGCCCAGCGTCTGCAGTCTATGCGTACAAGGAGACGTAGGGTCCGAGACCCATGGGGAAACTGGTGTGATGCAAAGGACTTAGAAGGACAAACGTTTGAGCATCTCTCTGCTGAGGAGTTGGCAAAAAGAAGAGAAGAGGAAAAAGGCAGACCTGTTAAATCTTCAAAAGTGCCAAGACCAACAAAAAGCTCGTTTGATCCCTTCCAACTGATACCTTGTAATTTTTTTAGTGAAGAAAAGCAGGAGCCATTTCAGGTGAAAGTGGCTTCAGAAGCACTTTTAATAATGGATTTGCATGCTCATGTTTCTATGGCAGAAGTGATTGGTCTGTTAGGAGGAAGATACTCAGAAGTTGATAAAGTAGTTGAAGTCTGTGCAGCAGAACCATGTAACAGTCTGAGTACAGGACTACAGTGTGAGATGGATCCTGTATCACAAACACAGGCCTCAGAAACCTTGGCTGTTAGAGGCTTCAGTGTTATTGGATGGTATCATTCTCATCCTGCTTTTGATCCTAATCCTTCCTTACGAGATATTGACACACAAGCTAAATACCAGAGTTACTTCTCCAGAGGAGGTGCAAAGTTCATTGGGATGATTGTTAGTCCCTATAATCGAAATAATCCCTTACCATATTCTCAGATTACCTGCCTGGTTATAAGTGAGGAAATTAGCCCAGATGGCTCTTATCGCTTACCTTACAAATTTGAAGTACAGCAGATGTTAGAAGAACCTCAGTGGGGATTAGTATTTGAAAAGACAAGATGGATAATAGAAAAATACAGGCTCTCCCATAGCAGCGTCCCCATGGATAAAATCTTTCGCCGGGATTCTGACCTGACTTGTTTGCAGAAACTTTTGGAGTGTATGAGGAAGACTCTGAGCAAAGTGACCAATTGCTTTATGGCTGAAGAATTCTTGACTGAAATAGAAAATTTGTTCCTTTCCAATTATAAAAGCAACCAAGAGAATGGAGTAACCGAAGAGAACTGTACAAAGGAATTGTTAATGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENRAVIEKMLLEEEYYLS
KKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQYFKNKVKCGLDKETPN
QKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEFIASDSQEALFSKSSR
GCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQMVEESHEEEELKPPEQ
EIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFGCEQAVYNRPQTVDKV
RIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSSKVPRPTKSSFDPFQLIPCNFFSEEKQEPFQV
KVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFDPNPSLRDIDTQAKYQ
SYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMDKIFRRDSDLTCLQKL
LECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSNQENGVTEENCTKELLM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33925
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L546S. Also contains the benign variation T264A and 3 silent nucleotide changes; A>C 594, C>T 735 and A>G 1275 bp.