cDNA Clone MYSM1

• Synonyms: 2A-DUB, 2ADUB
• Plasmid: pFB HTB MYSM1 S546L
• Parent Plasmid: pFastBac HTb
• Expressed: 6xHis-3C-MYSM1 S546L
• Genbank: NM_001085487.1
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTATGGCGGCTGAAGAGGCGGATGTGGATATCGAAGGGGACGTGGTAGCGGCGGCGGGGGCACAGCCAGGAAGTGGTGAAAATACAGCATCAGTTTTACAAAAAGATCACTATCTTGATTCATCTTGGAGAACAGAGAATGGCCTTATTCCTTGGACCTTGGATAACACCATCAGTGAAGAGAACAGAGCTGTTATTGAGAAAATGTTGTTGGAAGAAGAATATTATTTATCTAAAAAATCACAACCGGAAAAAGTCTGGCTTGATCAAAAGGAAGATGATAAAAAATACATGAAGAGTCTGCAGAAAACAGCAAAAATCATGGTACACTCTCCTACAAAACCAGCCAGTTACTCAGTAAAGTGGACGATAGAAGAAAAAGAGCTGTTTGAACAAGGGCTGGCTAAATTTGGCCGAAGATGGACCAAAATTTCAAAGCTAATTGGAAGCCGCACTGTTTTACAAGTGAAGAGTTATGCAAGACAGTATTTTAAAAATAAGGTCAAATGCGGTCTGGATAAAGAAACACCAAATCAGAAGACCGGCCATAATCTTCAAGTTAAAAATGAAGATAAAGGGACAAAGGCATGGACACCGTCATGTTTAAGGGGACGTGCTGATCCCAACTTGAATGCTGTAAAAATTGAAAAGTTATCTGATGATGAAGAAGTAGACATCACAGATGAGGTGGACGAGTTGTCTTCTCAAACACCCCAGAAGAATTCTAGCAGTGATCTTTTGTTAGACTTTCCTAATAGTAAAATGCATGAAACCAATCAAGGAGAATTCATTGCTTCTGACAGCCAGGAAGCTCTCTTTTCTAAGTCTTCCAGGGGCTGTCTTCAAAATGAAAAGCAAGATGAAACACTTTCAAGCTCAGAAATTACACTGTGGACTGAGAAACAGAGCAATGGTGACAAAAAATCAATTGAATTAAATGACCAGAAATTTAATGAATTGATTAAAAACTGCAACAAGCATGATGGAAGGGGAATAATAGTTGATGCCAGGCAGTTGCCTTCTCCAGAGCCTTGTGAAATTCAGAAAAATTTGAATGATAATGAAATGCTTTTTCATTCTTGCCAAATGGTAGAGGAAAGCCATGAGGAAGAAGAGCTTAAGCCACCAGAACAGGAAATAGAAATAGATAGAAATATCATTCAAGAAGAAGAAAAACAAGCAATTCCTGAGTTTTTTGAGGGGCGCCAAGCTAAAACACCAGAACGCTATTTGAAAATTAGAAATTATATTTTGGATCAATGGGAGATATGCAAACCGAAATACTTAAATAAGACCTCAGTACGTCCTGGCCTGAAGAACTGTGGAGATGTTAATTGTATTGGACGGATTCATACATACCTCGAATTGATAGGAGCAATCAATTTTGGATGTGAACAGGCTGTGTATAATAGGCCACAAACAGTTGACAAAGTACGAATCAGAGACAGAAAAGATGCAGTAGAAGCATACCAACTTGCCCAGCGTCTGCAGTCTATGCGTACAAGGAGACGTAGGGTCCGAGACCCATGGGGAAACTGGTGTGATGCAAAGGACTTAGAAGGACAAACGTTTGAGCATCTCTCTGCTGAGGAGTTGGCAAAAAGAAGAGAAGAGGAAAAAGGCAGACCTGTTAAATCTTCAAAAGTGCCAAGACCAACAAAAAGCTCGTTTGATCCCTTCCAACTGATACCTTGTAATTTTTTTAGTGAAGAAAAGCAGGAGCCATTTCAGGTGAAAGTGGCTTCAGAAGCACTTTTAATAATGGATTTGCATGCTCATGTTTCTATGGCAGAAGTGATTGGTCTGTTAGGAGGAAGATACTCAGAAGTTGATAAAGTAGTTGAAGTCTGTGCAGCAGAACCATGTAACAGTCTGAGTACAGGACTACAGTGTGAGATGGATCCTGTATCACAAACACAGGCCTCAGAAACCTTGGCTGTTAGAGGCTTCAGTGTTATTGGATGGTATCATTCTCATCCTGCTTTTGATCCTAATCCTTCCTTACGAGATATTGACACACAAGCTAAATACCAGAGTTACTTCTCCAGAGGAGGTGCAAAGTTCATTGGGATGATTGTTAGTCCCTATAATCGAAATAATCCCTTACCATATTCTCAGATTACCTGCCTGGTTATAAGTGAGGAAATTAGCCCAGATGGCTCTTATCGCTTACCTTACAAATTTGAAGTACAGCAGATGTTAGAAGAACCTCAGTGGGGATTAGTATTTGAAAAGACAAGATGGATAATAGAAAAATACAGGCTCTCCCATAGCAGCGTCCCCATGGATAAAATCTTTCGCCGGGATTCTGACCTGACTTGTTTGCAGAAACTTTTGGAGTGTATGAGGAAGACTCTGAGCAAAGTGACCAATTGCTTTATGGCTGAAGAATTCTTGACTGAAATAGAAAATTTGTTCCTTTCCAATTATAAAAGCAACCAAGAGAATGGAGTAACCGAAGAGAACTGTACAAAGGAATTGTTAATGTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENRAVIEKMLLEE
EYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQYFKNKVKCGLD
KETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEFIASDSQEALF
SKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQMVEESHEEEEL
KPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFGCEQAVYNRPQ
TVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSSKVPRPTKSSFDPFQLIPCNFFSEEKQ
EPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFDPNPSLRDIDT
QAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMDKIFRRDSDLT
CLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSNQENGVTEENCTKELLM*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU33916
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L546S. Also contains the benign variation T264A and 3 silent nucleotide changes; A>C 594, C>T 735 and A>G 1275 bp.; ; Estimated MW of expressed protein : 98348.05 Daltons