cDNA Clone NEK5

• Plasmid: pFB HTB NEK5
• Parent Plasmid: pFastBac HTb
• Expressed: 6xHis-NEK5
• Genbank: NM_199289.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATAAGTACGATGTGATTAAGGCCATCGGGCAAGGTGCCTTCGGGAAAGCATACTTAGCTAAAGGGAAATCAGATAGCAAGCACTGTGTCATAAAAGAGATCAATTTTGAAAAGATGCCCATACAAGAAAAAGAAGCTTCAAAGAAAGAAGTGATTCTTCTGGAAAAGATGAAACATCCCAACATTGTAGCCTTCTTCAATTCATTTCAAGAGAATGGCAGGCTGTTTATTGTAATGGAATATTGTGATGGAGGGGATCTCATGAAAAGGATCAATAGACAACGGGGTGTGTTATTTAGTGAAGATCAGATCCTCGGTTGGTTTGTACAGATTTCTCTAGGACTAAAACATATTCATGACAGGAAGATATTACACAGGGACATAAAAGCTCAGAACATTTTTCTTAGCAAGAACGGAATGGTGGCAAAGCTTGGGGACTTTGGTATAGCAAGAGTCCTGAATAATTCCATGGAACTTGCTCGAACTTGTATTGGAACACCTTACTACCTGTCCCCAGAGATCTGTCAGAATAAACCCTACAACAATAAAACGGATATTTGGTCTCTTGGCTGTGTCTTATATGAGCTCTGCACACTTAAACATCCTTTTGAGGGTAACAACTTACAGCAGCTGGTTCTGAAGATTTGTCAAGCACATTTTGCCCCAATATCTCCGGGGTTTTCTCGTGAGCTCCATTCCTTGATATCTCAGCTCTTTCAAGTATCTCCTCGAGACCGACCATCCATAAATTCCATTTTGAAAAGGCCCTTTTTAGAGAATCTTATTCCCAAATATTTGACTCCTGAGGTCATTCAGGAAGAATTCAGTCACATGCTTATATGCAGAGCAGGAGCGCCAGCTTCTCGACATGCTGGGAAGGTGGTCCAGAAGTGTAAAATACAAAAAGTGAGATTCCAGGGAAAGTGCCCACCAAGATCAAGGATATCTGTGCCAATTAAAAGGAATGCTATATTGCATAGAAATGAATGGAGACCACCAGCTGGAGCCCAGAAGGCCAGATCTATAAAAATGATAGAAAGACCCAAAATTGCTGCTGTCTGTGGACATTATGATTATTATTATGCTCAACTTGATATGCTGAGGAGGAGAGCCCACAAACCAAGTTATCACCCTATTCCTCAAGAAAATACTGGAGTTGAGGATTACGGTCAGGAAACGAGGCATGGTCCATCCCCAAGTCAATGGCCTGCTGAGTACCTTCAGAGAAAATTTGAAGCTCAACAATATAAGTTGAAAGTGGAGAAGCAATTGGGTCTTCGTCCATCTTCTGCCGAGCCAAATTACAACCAGAGACAAGAGCTAAGAAGTAATGGAGAAGAGCCTAGATTCCAGGAGCTGCCATTTAGGAAAAACGAAATGAAGGAACAGGAATATTGGAAGCAGTTAGAGGAAATACGCCAACAGTACCACAATGACATGAAAGAAATTAGAAAGAAGATGGGGAGAGAACCAGAGGAGAACTCAAAAATAAGTCATAAAACCTATTTGGTGAAGAAGAGTAACCTGCCTGTCCATCAAGATGCATCTGAGGGAGAAGCACCTGTGCAGATGGAATTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCACGATCTTGGCTCACCGCAACCTCCGCCTCCCAGGACATTGAAAAAGACTTGAAACAAATGAGGCTTCAGAACACAAAGGAAAGTAAAAATCCAGAACAGAAATATAAAGCTAAGAAGGGGGTAAAATTTGAAATTAATTTAGACAAATGTATTTCTGATGAAAACATCCTCCAAGAGGAAGAGGCAATGGATATACCAAATGAAACTTTGACCTTTGAGGATGGCATGAAGTTTAAGGAATATGAATGTGTAAAGGAGCATGGAGATTATACAGACAAAGCATTTGAAAAACTTCACTGCCCAGAAGCAGGGTTTTCCACGCAGACTGTAGCTGCTGTGGGAAACAGGAGGCAGTGGGATGGAGGAGCGCCTCAGACTCTGCTGCAGATGATGGCAGTGGCCGACATCACCTCCACCTGCCCCACGGGGCCTGACAGTGAGTCTGTGCTTAGCGTCAGTCGTCAGGAAGGGAAGACCAAGGACCCGTACAGCCCAGTGCTCATCCTGATGTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDKYDVIKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSFQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYLTPEVIQEEFSHMLICRAGAPASRHAGKVVQKCKIQKVRFQGKCPPRSRISVPIKRNAILHRNEWRPPAGAQKARSIKMIERPKIAAVCGHYDYYYAQLDMLRRRAHKPSYHPIPQENTGVEDYGQETRHGPSPSQWPAEYLQRKFEAQQYKLKVEKQLGLRPSSAEPNYNQRQELRSNGEEPRFQELPFRKNEMKEQEYWKQLEEIRQQYHNDMKEIRKKMGREPEENSKISHKTYLVKKSNLPVHQDASEGEAPVQMEFRSCCPGWSAMARSWLTATSASQDIEKDLKQMRLQNTKESKNPEQKYKAKKGVKFEINLDKCISDENILQEEEAMDIPNETLTFEDGMKFKEYECVKEHGDYTDKAFEKLHCPEAGFSTQTVAAVGNRRQWDGGAPQTLLQMMAVADITSTCPTGPDSESVLSVSRQEGKTKDPYSPVLILM

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU33483
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 84817.39 Daltons