cDNA Clone GLG1p (yeast)

• Plasmid: pGEX6P-1-Glg1p yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST Glg1p yeast
• Genbank: U25546.1
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGGGAATGTATAAGAAGCTGGCTATTGCCACATTGCTCTATTC
CGCAGATTATTTACCAGGTGTGTTTGCCCTTGGTCACCAAGTGAATAAAT
TGTTAGAGGAAGCAGGCAAAAAAGGCGACATTGAAACATGCCTTATTGTG
ACGACCTCTCTATTTAATGACACTTTAAGCGAGTTAGCCAAGAATCTTTT
ACAATCAATATACACCAAAATTGTGTTGGTAGAGCCTTTAGATTGCCAAG
AAGAAAGCATACAGAAGAATAGTGAGAATTTAGCTCTTTTGGAAAGACCT
GAATTATCCTTTGCCTTAATCAAAGCGAGACTATGGGAGTTAACTCAGTT
CGAACAAGTTCTTTACTTGGATTCCGACACTTTACCTCTGAATAAAGAAT
TTTTAAAGTTGTTTGACATTATGTCTAAACAAACCACGTCACAAGTGGGT
GCTATTGCTGATATCGGCTGGCCTGATATGTTCAATAGCGGTGTCATGAT
GCTAATACCGGATGCTGATACTGCATCCGTTTTACAAAATTATATTATTG
AAAACACTTCAATTGATGGTTCTGACCAGGGCATTTTAAACCAGTTCTTC
AATCAAAATTGTTGCACTGATGAGCTAGTTAAAGACAGTTTCTCTCGAGA
ATGGGTACAGTTATCATTTACGTATAACGTAACTATTCCTAATTTAGGCT
ACCAATCTTCACCTGCTATGAATTACTTTAAACCTAGCATTAAGTTGATT
CATTTCATTGGGAAACACAAGCCATGGTCATTGTGGTCCCAAAAGAATTT
CATTAAAAACGAGTATCATGATCAATGGAATGAGGTATACGAGGAATTTA
AGGAAGAACATCAATTAAATAACGAAGTTTCGAAGCCAAAGATTAGTGAT
TCCGATAAAACTGAGACTCCTGAAACGATAACCCCAGTGTATGCCCCTCC
ATCGAACGAACCCACTACGAATCAAGAGATCGACACAATTAGTACAGTGG
AAGAAAACGTGGACAACCAGAACGCCGAGCCTGTTCCTAACTCGGATCAT
AGCCCTGCTCCTAACCCTGTCCCATTGGATTTTACCAAGTGGTTAACAAC
CTTTATTAACAAAGATCACTTGACTAATCAGCCAGTAAATGAAAGCAGAG
AATATTCGAAAGAGAATGATAACAATATAATCAATAGCAGTTCAGACCGC
GACCAAGAAAGTCCTCCAAATAGTACTCAGGAACTAAACAGCTCTTATAG
CGTTGTGAGTACTCAAGCTGATAGCGATGAGCACCAAAATGCAGAAGAGG
AGGATAGTACGACAGATAACGCTTCAAATTCTGGAGAAGAAAGCCACCTC
GATGATATAAGCACGGCCGCTTCTTCCAATAACAATGTAAGTAATCAAGC
GGATGACAAAAATTTTAGCAATTCTAAAGAAAATAATATCAGTGTCGAAT
CTAGCCCTTCAAATCCAGAGCAGAAAAGGTCAACTGACAATATACAAAAA
CCAAGTGTTTCTACTAATGACTTACCAGACGACGTAGAACCACATACTTC
TGTTGATGACAATATACAGTACTTAGAAAAAGACAAGGAAGGTTATGAAG
AATTTCTTCCGGACGTGTACGAGTCAAATGCAATCGATAACGAAGAAGAA
TTTTTTGATGACGATGCAAGAGATGCTACCGAAGGAGAAACGAAGACGAG
TGCAGTTGCAGATAAACAGGAGGATATGAAACTTACAGCAGAGGAAACCA
ACCAACCGCAACAAGAAATGCCCAACTTCAAATTTGACTGGGAGGACTCT
GATTATCTATCCAAAGTAGAGAGGTGTTTCCCTGATGATATTTTTGAGTA
TGCAGTAGAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGMYKKLAIATLLYSADYL
PGVFALGHQVNKLLEEAGKKGDIETCLIVTTSLFNDTLSELAKNLLQSIY
TKIVLVEPLDCQEESIQKNSENLALLERPELSFALIKARLWELTQFEQVL
YLDSDTLPLNKEFLKLFDIMSKQTTSQVGAIADIGWPDMFNSGVMMLIPD
ADTASVLQNYIIENTSIDGSDQGILNQFFNQNCCTDELVKDSFSREWVQL
SFTYNVTIPNLGYQSSPAMNYFKPSIKLIHFIGKHKPWSLWSQKNFIKNE
YHDQWNEVYEEFKEEHQLNNEVSKPKISDSDKTETPETITPVYAPPSNEP
TTNQEIDTISTVEENVDNQNAEPVPNSDHSPAPNPVPLDFTKWLTTFINK
DHLTNQPVNESREYSKENDNNIINSSSDRDQESPPNSTQELNSSYSVVST
QADSDEHQNAEEEDSTTDNASNSGEESHLDDISTAASSNNNVSNQADDKN
FSNSKENNISVESSPSNPEQKRSTDNIQKPSVSTNDLPDDVEPHTSVDDN
IQYLEKDKEGYEEFLPDVYESNAIDNEEEFFDDDARDATEGETKTSAVAD
KQEDMKLTAEETNQPQQEMPNFKFDWEDSDYLSKVERCFPDDIFEYAVE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; Differs to genbank at I64L, N79D, F181I, D312Y,N397D, P465A, G467D. Insert amplified from W3031a in 3 separate reactions gave same sequence. Thought to be strain difference to genbank reference and all annotated as variations on ensembl; ; Estimated MW of expressed protein : 96840 Daltons