cDNA Clone GLG2p (yeast)

• Plasmid: pGEX6P-1-Glg2p yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST Glg2p yeast
• Genbank: AAA91644.1
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGGCCAAGAAAGTTGCCATCTGTACATTGCTGTATTCACGGGA
CTATTTACCTGGCGCATTGACTTTAGCATACCAATTACAAAAACTCTTGA
AACACGCTGTAGTGGAAGATGAGATAACCTTATGCCTGCTAATTGAAAAA
AAATTGTTTGGAGATGAGTTTAAACCTCAGGAAATAGCTTTAATTAGAAG
TCTTTTTAAAGAGATTATCATTATTGAACCGCTAAAGGATCAAGAGAAGA
GCATAGAGAAGAATAAGGCAAATCTTGAACTGTTGAAAAGGCCTGAGCTA
TCTCATACTTTACTCAAGGCTAGACTGTGGGAACTGGTGCAGTTCGATCA
GGTTTTATTTTTAGATGCTGATACTTTACCATTAAATAAAGAATTTTTTG
AAATCCTACGATTATACCCTGAACAAACAAGATTCCAGATTGCTGCGGTT
CCAGACATCGGATGGCCTGACATGTTCAACACCGGTGTACTACTACTAAT
TCCTGATTTAGATATGGCAACAAGTTTACAAGATTTTTTGATTAAAACTG
TATCGATTGATGGTGCTGATCAAGGAATCTTCAATCAATTTTTTAATCCT
ATTTGCAACTACAGCAAAGAGGTTTTGCATAAGGTGTCTCCACTCATGGA
ATGGATACGCCTCCCCTTCACCTACAACGTAACCATGCCCAACTACGGAT
ACCAGTCTTCACCAGCCATGAATTTTTTCCAACAACACATCAGACTTATT
CACTTCATTGGAACGTTTAAGCCATGGTCCCGTAATACAACTGACTATGA
CGACCACTATTACCAGCTGTGGAGAAGTACACAGCGTGAACTTTATAGTG
AATGCCACTTAAGTAACTACTTCACACACTTGCAATTGGGCAATATTGAA
ACAGAGACAAATTTTTACCACGAACCTCCTTGTCTTCAAGACCTGTTGAA
TCATGGTAAAAGAGAAAATCAAAAGCACGTTGACCTGGATATAACCTCAG
TAGATCGAAATGCTTCGCAGAAGAGTACTGCAGAGAAACATGATATTGAG
AAACCGACATCTAAGCCGCAATCTGCTTTTAAATTTGATTGGGAGTCTAC
GGATTATTTAGACCGCGTCCAAAGAGCATTCCCAAAGCCTGATACCTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAKKVAICTLLYSRDYLPG
ALTLAYQLQKLLKHAVVEDEITLCLLIEKKLFGDEFKPQEIALIRSLFKE
IIIIEPLKDQEKSIEKNKANLELLKRPELSHTLLKARLWELVQFDQVLFL
DADTLPLNKEFFEILRLYPEQTRFQIAAVPDIGWPDMFNTGVLLLIPDLD
MATSLQDFLIKTVSIDGADQGIFNQFFNPICNYSKEVLHKVSPLMEWIRL
PFTYNVTMPNYGYQSSPAMNFFQQHIRLIHFIGTFKPWSRNTTDYDDHYY
QLWRSTQRELYSECHLSNYFTHLQLGNIETETNFYHEPPCLQDLLNHGKR
ENQKHVDLDITSVDRNASQKSTAEKHDIEKPTSKPQSAFKFDWESTDYLD
RVQRAFPKPDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35449
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 71368 Daltons