cDNA Clone RPS6KL1

• Plasmid: pGEX6P-1-RPS6KL1
• Parent Plasmid: pGEX6P1
• Expressed: GST RPS6KL1
• Genbank: BC004540.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAGCCTGGTGGCCTGTGAGTGCCTGCCCAGCCCCGGCCTGGA
GCCTGAGCCTTGCTCACGAGCACGGTCCCAAGCTCACGTGTACCTGGAGC
AGATTCGCAACAGGGTGGCTCTGGGAGTGCCTGACATGACAAAACGTGAC
TATCTGGTGGATGCGGCCACGCAGATCCGGCTGGCCCTGGAGCGCGATGT
TAGTGAGGACTATGAGGCGGCCTTCAACCACTATCAGAATGGCGTGGACG
TGCTGCTCCGTGGCATACACGTTGACCCCAACAAGGAGCGACGTGAGGCT
GTGAAGCTGAAAATTACCAAATACCTGCGGCGGGCAGAGGAGATCTTCAA
CTGCCACCTGCAGCGGCCGCTGAGCAGTGGAGCCAGCCCCAGCGCGGTGC
AGCTGGTCCAGGACCCGGCAACCGGAGGGACCTTTGTGGTGAAGAGCCTA
CCCAGGTGCCACATGGTGAGCAGGGAGCGGCTGACCATCATCCCACACGG
AGTCCCCTACATGACGAAGCTGCTCAGGTACTTTGTGAGCGAGGACTCCA
TCTTCCTGCACCTGGAGCATGTGCAAGGAGGCACTCTCTGGTCCCACCTG
CTCTCCCAGGCGCACTCCCGACATTCTGGGCTCAGCTCTGGCTCTACCCA
GGAGAGGATGAAGGCTCAGCTCAACCCCCACCTCAACCTCCTGACCCCAG
CGAGGCTTCCCTCAGGCCATGCCCCTGGCCAGGACAGAATCGCCCTGGAG
CCTCCTAGGACTTCTCCGAACCTTCTCCTAGCTGGGGAGGCCCCATCCAC
CAGACCCCAGAGGGAGGCTGAAGGTGAACCCACAGCCAGGACCAGCACCT
CTGGCTCCTCGGACCTTCCAAAGGCCCCAGGTGGCCACCTGCACCTTCAA
GCTAGGAGGGCTGGCCAGAACTCAGACGCTGGGCCCCCTCGGGGGCTCAC
TTGGGTTCCTGAGGGGGCCGGCCCGGTGCTAGGGGGCTGTGGCCGAGGCA
TGGATCAGAGCTGCCTGTCAGCAGATGGGGCCGGCCGGGGCTGTGGCAGG
GCCACCTGGAGTGTGAGAGAGGAGCAGGTGAAGCAGTGGGCGGCAGAGAT
GCTGGTAGCGCTGGAGGCGCTGCACGAGCAGGGGGTGCTGTGCCGGGACC
TCCACCCCGGGAACCTGCTCCTGGACCAGGCAGGTCACATCCGGCTCACA
TATTTTGGCCAGTGGTCAGAGGTGGAGCCCCAGTGCTGCGGGGAGGCCGT
GGACAATCTCTACAGCGCCCCAGAGGTGGGTGGGATTTCCGAGCTGACGG
AAGCCTGTGACTGGTGGAGCTTTGGGTCTCTACTGTATGAACTGCTGACG
GGAATGGCACTGTCCCAGAGCCACCCTTCAGGAATCCAGGCCCACACCCA
GCTCCAGCTGCCCGAGTGGCTCAGTCGCCCAGCGGCCTCTCTGCTGACTG
AGCTGCTGCAGTTCGAGCCTACCCGGCGCCTGGGCATGGGAGAAGGTGGT
GTCAGCAAACTCAAGTCCCATCCCTTTTTCAGTACCATCCAATGGAGCAA
GCTGGTGGGGACATCTCTGGAGACTCATCTCAGGACACTGATCCACTGGC
TCAGTGGACCCAAACCAGACTGTCCTGGCTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLVACECLPSPGLEPEPC
SRARSQAHVYLEQIRNRVALGVPDMTKRDYLVDAATQIRLALERDVSEDY
EAAFNHYQNGVDVLLRGIHVDPNKERREAVKLKITKYLRRAEEIFNCHLQ
RPLSSGASPSAVQLVQDPATGGTFVVKSLPRCHMVSRERLTIIPHGVPYM
TKLLRYFVSEDSIFLHLEHVQGGTLWSHLLSQAHSRHSGLSSGSTQERMK
AQLNPHLNLLTPARLPSGHAPGQDRIALEPPRTSPNLLLAGEAPSTRPQR
EAEGEPTARTSTSGSSDLPKAPGGHLHLQARRAGQNSDAGPPRGLTWVPE
GAGPVLGGCGRGMDQSCLSADGAGRGCGRATWSVREEQVKQWAAEMLVAL
EALHEQGVLCRDLHPGNLLLDQAGHIRLTYFGQWSEVEPQCCGEAVDNLY
SAPEVGGISELTEACDWWSFGSLLYELLTGMALSQSHPSGIQAHTQLQLP
EWLSRPAASLLTELLQFEPTRRLGMGEGGVSKLKSHPFFSTIQWSKLVGT
SLETHLRTLIHWLSGPKPDCPG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35301
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 85988 Daltons