cDNA Clone TNF alpha

• Plasmid: pEGFP-N1-TNF alpha
• Parent Plasmid: pEGFPN1
• Expressed: TNF alpha GFP
• Genbank: NP_000585.2
• Tag: GFP
• Species: Human

Sequence of Insert

GTCGACATGAGCACTGAAAGCATGATCCGGGACGTGGAGCTGGCCGAGGA
GGCGCTCCCCAAGAAGACAGGGGGGCCCCAGGGCTCCAGGCGGTGCTTGT
TCCTCAGCCTCTTCTCCTTCCTGATCGTGGCAGGCGCCACCACGCTCTTC
TGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGGGAAGAGTTCCCCAG
GGACCTCTCTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCTTCTC
GAACCCCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCT
GAGGGGCAGCTCCAGTGGCTGAACCGCCGGGCCAATGCCCTCCTGGCCAA
TGGCGTGGAGCTGAGAGATAACCAGCTGGTGGTGCCATCAGAGGGCCTGT
ACCTCATCTACTCCCAGGTCCTCTTCAAGGGCCAAGGCTGCCCCTCCACC
CATGTGCTCCTCACCCACACCATCAGCCGCATCGCCGTCTCCTACCAGAC
CAAGGTCAACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAGACCC
CAGAGGGGGCTGAGGCCAAGCCCTGGTATGAGCCCATCTATCTGGGAGGG
GTCTTCCAGCTGGAGAAGGGTGACCGACTCAGCGCTGAGATCAATCGGCC
CGACTATCTCGACTTTGCCGAGTCTGGGCAGGTCTACTTTGGGATCATTG
CCCTGTCACGGGATCC

Amino Acid Sequence

MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCL
LHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEG
QLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHV
LLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVF
QLEKGDRLSAEINRPDYLDFAESGQVYFGIIALSRDPPVATMVSKGEELF
TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWP
TLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
NGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS
KDPNEKRDHMVLLEFVTAAGITLGMDELYK

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU35226
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C Ter GFP for mammalian expression; ; Estimated MW of expressed protein : 53390 Daltons