cDNA Clone HCK

• Plasmid: pGEX6P-2-HCK
• Parent Plasmid: pGEX6P2
• Expressed: GST HCK
• Genbank: NP_001165600.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGGGGGGCGCTCAAGCTGCGAGGATCCGGGCTGCCCGCGAGA
CGAGGAGCGGGCGCCCAGGATGGGGTGCATGAAGTCCAAGTTCCTCCAGG
TCGGAGGCAATACATTCTCAAAAACTGAAACCAGCGCCAGCCCACACTGT
CCTGTGTACGTGCCGGATCCCACATCCACCATCAAGCCGGGGCCTAATAG
CCACAACAGCAACACACCAGGAATCAGGGAGGCAGGCTCTGAGGACATCA
TCGTGGTTGCCCTGTATGATTACGAGGCCATTCACCACGAAGACCTCAGC
TTCCAGAAGGGGGACCAGATGGTGGTCCTAGAGGAATCCGGGGAGTGGTG
GAAGGCTCGATCCCTGGCCACCCGGAAGGAGGGCTACATCCCAAGCAACT
ATGTCGCCCGCGTTGACTCTCTGGAGACAGAGGAGTGGTTTTTCAAGGGC
ATCAGCCGGAAGGACGCAGAGCGCCAACTGCTGGCTCCCGGCAACATGCT
GGGCTCCTTCATGATCCGGGATAGCGAGACCACTAAAGGAAGCTACTCTT
TGTCCGTGCGAGACTACGACCCTCGGCAGGGAGATACCGTGAAACATTAC
AAGATCCGGACCCTGGACAACGGGGGCTTCTACATATCCCCCCGAAGCAC
CTTCAGCACTCTGCAGGAGCTGGTGGACCACTACAAGAAGGGGAACGACG
GGCTCTGCCAGAAACTGTCGGTGCCCTGCATGTCTTCCAAGCCCCAGAAG
CCTTGGGAGAAAGATGCCTGGGAGATCCCTCGGGAATCCCTCAAGCTGGA
GAAGAAACTTGGAGCTGGGCAGTTTGGGGAAGTCTGGATGGCCACCTACA
ACAAGCACACCAAGGTGGCAGTGAAGACGATGAAGCCAGGGAGCATGTCG
GTGGAGGCCTTCCTGGCAGAGGCCAACGTGATGAAAACTCTGCAGCATGA
CAAGCTGGTCAAACTTCATGCGGTGGTCACCAAGGAGCCCATCTACATCA
TCACGGAGTTCATGGCCAAAGGAAGCTTGCTGGACTTTCTGAAAAGTGAT
GAGGGCAGCAAGCAGCCATTGCCAAAACTCATTGACTTCTCAGCCCAGAT
TGCAGAAGGCATGGCCTTCATCGAGCAGAGGAACTACATCCACCGAGACC
TCCGAGCTGCCAACATCTTGGTCTCTGCATCCCTGGTGTGTAAGATTGCT
GACTTTGGCCTGGCCCGGGTCATTGAGGACAACGAGTACACGGCTCGGGA
AGGGGCCAAGTTCCCCATCAAGTGGACAGCTCCTGAAGCCATCAACTTTG
GCTCCTTCACCATCAAGTCAGACGTCTGGTCCTTTGGTATCCTGCTGATG
GAGATCGTCACCTACGGCCGGATCCCTTACCCAGGGATGTCAAACCCTGA
AGTGATCCGAGCTCTGGAGCGTGGATACCGGATGCCTCGCCCAGAGAACT
GCCCAGAGGAGCTCTACAACATCATGATGCGCTGCTGGAAAAACCGTCCG
GAGGAGCGGCCGACCTTCGAATACATCCAGAGTGTGCTGGATGACTTCTA
CACGGCCACAGAGAGCCAGTACCAACAGCAGCCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAGGRSSCED
PGCPRDEERAPRMGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTI
KPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLE
ESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLL
APGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFY
ISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPR
ESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVM
KTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLI
DFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDN
EYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYP
GMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQS
VLDDFYTATESQYQQQP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35122
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 87269 Daltons