cDNA Clone garA

• Plasmid: GST garA
• Parent Plasmid: pGEX6P1
• Expressed: GST garA
• Genbank: CP001658.1
• Tag: GST
• Species: Mycobacterium tuberculosis

Sequence of Insert

GGATCGGTGACGGACATGAACCCGGATATTGAGAAGGACCAGACCTCCGA
TGAAGTCACGGTAGAGACGACCTCCGTCTTCCGCGCAGACTTCCTCAGCG
AGCTGGACGCTCCTGCGCAAGCGGGTACGGAGAGCGCGGTCTCCGGGGTG
GAAGGGCTCCCGCCGGGCTCGGCGTTGCTGGTAGTCAAACGAGGCCCCAA
CGCCGGGTCCCGGTTCCTACTCGACCAAGCCATCACGTCGGCTGGTCGGC
ATCCCGACAGCGACATATTTCTCGACGACGTGACCGTGAGCCGTCGCCAT
GCTGAATTCCGGTTGGAAAACAACGAATTCAATGTCGTCGATGTCGGGAG
TCTCAACGGCACCTACGTCAACCGCGAGCCCGTGGATTCGGCGGTGCTGG
CGAACGGCGACGAGGTCCAGATCGGCAAGTTCCGGTTGGTGTTCTTGACC
GGACCCAAGCAAGGCGAGGATGACGGGAGTACCGGGGGCCCGTGACTCGA
G

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTDMNPDIEKDQTSDEVTV
ETTSVFRADFLSELDAPAQAGTESAVSGVEGLPPGSALLVVKRGPNAGSR
FLLDQAITSAGRHPDSDIFLDDVTVSRRHAEFRLENNEFNVVDVGSLNGT
YVNREPVDSAVLANGDEVQIGKFRLVFLTGPKQGEDDGSTGGP*

• Vector Type: Other
• DU Number: DU35019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 44041 Daltons