cDNA Clone SIK

• Plasmid: pcDNA5-FRT/TO SIK FLAG
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SIK FLAG
• Genbank: NM_173354
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGG
TCAGGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGA
CCCTGGGCAAAGGCAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTC
ACCAAAACGCAGGTTGCAATAAAAATAATTGATAAAACACGATTAGATTC
AAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGCTTCTGA
ACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATG
CTTTACATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTT
GACTTCCAACGGGCACCTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGC
AAATCCTGTCGGCCGTGGAGTACTGTCACGACCATCACATCGTCCACCGG
GACCTCAAGACCGAGAACCTCCTGCTGGATGGCAACATGGACATCAAGCT
GGCAGATTTTGGATTTGGGAATTTCTACAAGTCAGGAGAGCCTCTGTCCA
CGTGGTGTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAG
GAGTATGAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTA
CGTCCTGGTCTGCGGTTCTCTCCCCTTCGATGGGCCTAACCTGCCGACGC
TGAGACAGCGGGTGCTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCT
CAAGACTGTGAGAGCCTGATCCGCCGCATGCTGGTGGTGGACCCCGCCAG
GCGCATCACCATCGCCCAGATCCGGCAGCACCGGTGGATGCGGGCTGAGC
CCTGCTTGCCGGGACCCGCCTGCCCCGCCTTCTCCGCACACAGCTACACC
TCCAACCTGGGCGACTACGATGAGCAGGCGCTGGGTATCATGCAGACCCT
GGGCGTGGACCGGCAGAGGACGGTGGAGTCACTGCAAAACAGCAGCTATA
ACCACTTTGCTGCCATTTATTACCTCCTCCTTGAGCGGCTCAAGGAGTAT
CGGAATGCCCAGTGCGCCCGCCCCGGGCCTGCCAGGCAGCCGCGGCCTCG
GAGCTCGGACCTCAGTGGTTTGGAGGTGCCTCAGGAAGGTCTTTCCACCG
ACCCTTTCCGACCTGCCTTGCTGTGCCCGCAGCCGCAGACCTTGGTGCAG
TCCGTCCTCCAGGCCGAGATGGACTGTGAGCTCCAGAGCTCGCTGCAGTG
GCCCTTGTTCTTCCCGGTGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCC
GGCCCGTGTCCCCAAGCAGCCTGCTGGACACAGCCATCAGTGAGGAGGCC
AGGCAGGGGCCGGGCCTAGAGGAGGAGCAGGACACGCAGGAGTCCCTGCC
CAGCAGCACGGGCCGGAGGCACACCCTGGCCGAGGTCTCCACCCGCCTCT
CCCCACTCACCGCGCCATGTATAGTCGTCTCCCCCTCCACCACGGCAAGT
CCTGCAGAGGGAACCAGCTCTGACAGTTGTCTGACCTTCTCTGCGAGCAA
AAGCCCCGCGGGGCTCAGTGGCACCCCGGCCACTCAGGGGCTGCTGGGCG
CCTGCTCCCCGGTCAGGCTGGCCTCGCCCTTCCTGGGGTCGCAGTCCGCC
ACCCCAGTGCTGCAGGCTCAGGGGGGCTTGGGAGGAGCTGTTCTGCTCCC
TGTCAGCTTCCAGGAGGGACGGCGGGCGTCGGACACCTCACTGACTCAAG
GGCTGAAGGCCTTTCGGCAGCAGCTGAGGAAGACCACGCGGACCAAAGGG
TTTCTGGGACTGAACAAAATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGC
CCCCGCCAGCCGGGCCAGCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTG
CACAGAGCCCAGGCCTGCACGGCGGCGCAGCCGGCAGCCGGGAGGGCTGG
AGCCTGCTGGAGGAGGTGCTAGAGCAGCAGAGGCTGCTCCAGTTACAGCA
CCACCCGGCCGCTGCACCCGGCTGCTCCCAGGCCCCCCAGCCGGCCCCTG
CCCCGTTTGTGATCGCCCCCTGTGATGGCCCTGGGGCTGCCCCGCTCCCC
AGCACCCTCCTCACGTCGGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCA
GACCGGCGCGTCCCCGGTGGCCTCAGCGGCGCAGCTCCTGGACACACACC
TGCACATTGGCACCGGCCCCACCGCCCTCCCCGCTGTGCCCCCACCACGC
CTGGCCAGGCTGGCCCCAGGTTGTGAGCCCCTGGGGCTGCTGCAGGGGGA
CTGTGAGATGGAGGACCTGATGCCCTGCTCCCTAGGCACGTTTGTCCTGG
TGCAGGACTACAAGGACGATGACGATAAGTAAGCGGCCGC

Amino Acid Sequence

MVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTK
TQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLY
IVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHDHHIVHRDL
KTENLLLDGNMDIKLADFGFGNFYKSGEPLSTWCGSPPYAAPEVFEGKEY
EGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQD
CESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSN
LGDYDEQALGIMQTLGVDRQRTVESLQNSSYNHFAAIYYLLLERLKEYRN
AQCARPGPARQPRPRSSDLSGLEVPQEGLSTDPFRPALLCPQPQTLVQSV
LQAEMDCELQSSLQWPLFFPVDASCSGVFRPRPVSPSSLLDTAISEEARQ
GPGLEEEQDTQESLPSSTGRRHTLAEVSTRLSPLTAPCIVVSPSTTASPA
EGTSSDSCLTFSASKSPAGLSGTPATQGLLGACSPVRLASPFLGSQSATP
VLQAQGGLGGAVLLPVSFQEGRRASDTSLTQGLKAFRQQLRKTTRTKGFL
GLNKIKGLARQVCQAPASRASRGGLSPFHAPAQSPGLHGGAAGSREGWSL
LEEVLEQQRLLQLQHHPAAAPGCSQAPQPAPAPFVIAPCDGPGAAPLPST
LLTSGLPLLPPPLLQTGASPVASAAQLLDTHLHIGTGPTALPAVPPPRLA
RLAPGCEPLGLLQGDCEMEDLMPCSLGTFVLVQDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32718
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter FLAG for mammalian expression; ; Estimated MW of expressed protein : 85893 Daltons