cDNA Clone MST4

• Plasmid: pCMV-FLAG-1 MST4
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG MST4
• Genbank: NP_057626
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCCCACTCGCCGGTGGCTGTCCAAGTGCCTGGGATGCAGAA
TAACATAGCTGATCCAGAAGAACTGTTCACAAAATTAGAGCGCATTGGGA
AAGGCTCATTTGGGGAAGTTTTCAAAGGAATTGATAACCGTACCCAGCAA
GTCGTTGCTATTAAAATCATAGACCTTGAGGAAGCCGAAGATGAAATAGA
AGACATTCAGCAAGAAATAACTGTCTTGAGTCAATGTGACAGCTCATATG
TAACAAAATACTATGGGTCATATTTAAAGGGGTCTAAATTATGGATAATA
ATGGAATACCTGGGCGGTGGTTCAGCACTGGATCTTCTTCGAGCTGGTCC
ATTTGATGAGTTCCAGATTGCTACCATGCTAAAGGAAATTTTAAAAGGTC
TGGACTATCTGCATTCAGAAAAGAAAATTCACCGAGACATAAAAGCTGCC
AATGTCTTGCTCTCAGAACAAGGAGATGTTAAACTTGCTGATTTTGGAGT
TGCTGGTCAGCTGACAGATACACAGATTAAAAGAAATACCTTTGTGGGAA
CTCCATTTTGGATGGCTCCTGAAGTTATTCAACAGTCAGCTTATGACTCA
AAAGCTGACATTTGGTCATTGGGAATTACTGCTATTGAACTAGCCAAGGG
AGAGCCACCTAACTCCGATATGCATCCAATGAGAGTTCTGTTTCTTATTC
CCAAAAACAATCCTCCAACTCTTGTTGGAGACTTTACTAAGTCTTTTAAG
GAGTTTATTGATGCTTGCCTGAACAAAGATCCATCATTTCGTCCTACAGC
AAAAGAACTTCTGAAACACAAATTCATTGTAAAAAATTCAAAGAAGACTT
CTTATCTGACTGAACTGATAGATCGTTTTAAGAGATGGAAGGCAGAAGGA
CACAGTGATGATGAATCTGATTCCGAGGGCTCTGATTCGGAATCTACCAG
CAGGGAAAACAATACTCATCCTGAATGGAGCTTTACCACCGTACGAAAGA
AGCCTGATCCAAAGAAAGTACAGAATGGGGCAGAGCAAGATCTTGTGCAA
ACCCTGAGTTGTTTGTCTATGATAATCACACCTGCATTTGCTGAACTTAA
ACAGCAGGACGAGAATAACGCTAGCAGGAATCAGGCGATTGAAGAACTCG
AGAAAAGTATTGCTGTGGCTGAAGCCGCCTGTCCCGGCATCACAGATAAA
ATGGTGAAGAAACTAATTGAAAAATTTCAAAAGTGTTCAGCAGACGAATC
CCCCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAHSPVAVQVPGMQNNIADPEELFTKLERIGKGSFGEVF
KGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSSYVTKYYGSY
LKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEK
KIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPE
VIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTL
VGDFTKSFKEFIDACLNKDPSFRPTAKELLKHKFIVKNSKKTSYLTELID
RFKRWKAEGHSDDESDSEGSDSESTSRENNTHPEWSFTTVRKKPDPKKVQ
NGAEQDLVQTLSCLSMIITPAFAELKQQDENNASRNQAIEELEKSIAVAE
AACPGITDKMVKKLIEKFQKCSADESP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32373
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; ; Estimated MW of expressed : 47797 Daltons; ; All MST4 plasmids MUST be grown at 30 degrees C or less to prevent recombination.