cDNA Clone GCK

• Synonyms: MAP4K2; BL44; GCK; RAB8IP
• Plasmid: pCMV-FLAG-1 GCK (with M220V L796R)
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG GCK
• Genbank: NM_004579.4* see notes
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTTCGAGCTGCTGCAGCGTGTGGGGGCCGGGACCTATGGCGACGTCTACAAGGCCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTAGACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAGGAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGTCTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGATCCACAGAGACATCAAGGGGGCCAACCTTCTCCTCACTCTCCAGGGAGATGTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGCCAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCCCTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCACCTGCACCCCGTGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGCCGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTCTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAAGCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCACACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCTGAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTCCCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTCACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATGAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTATTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATGGGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCCAGCAGAGGAGCCTCTGTCCAGTCCCCCAGGAACCCTGCCCCCACCTCCTTCAGGCCCCAACAGCTCCCCACTGCTGCCCACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCTGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGCCCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAGGATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCCACAGAGACATCATCCGGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAgcggccgc

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECRHPNVVAYIGSYLRN
DRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEVA
AVERKGGYNELCDVWALGITAIELGELQPPLFHLHPVRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLT
QLLDKASDPHLGTPSPEDCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTI
RSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEPLSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNGCPL
RIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIP
RRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVL
PLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETR
IFRVLGAHRDIIRESIPTDNPEAHSNLYILTGHQSTY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32372
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Changes in this clone compared with reference: M220V L796R. For equivalent clone with these changes back-mutated to wild-type use DU58719.
• Low Temp: No