cDNA Clone DAPK3

• Plasmid: pGEX6P-1-DAPK3
• Parent Plasmid: pGEX6P1
• Expressed: GST DAPK3
• Genbank: NP_001339.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCCACGTTCAGGCAGGAGGACGTGGAGGACCATTATGAGAT
GGGGGAGGAGCTGGGCAGCGGCCAGTTTGCGATCGTGCGGAAGTGCCGGC
AGAAGGGCACGGGCAAGGAGTACGCAGCCAAGTTCATCAAGAAGCGCCGC
CTGTCATCCAGCCGGCGTGGGGTGAGCCGGGAGGAGATCGAGCGGGAGGT
GAACATCCTGCGGGAGATCCGGCACCCCAACATCATCACCCTGCACGACA
TCTTCGAGAACAAGACGGACGTGGTCCTCATCCTGGAGCTGGTCTCTGGC
GGGGAGCTCTTTGACTTCCTGGCGGAGAAGGAGTCGCTGACGGAGGACGA
GGCCACCCAGTTCCTCAAGCAGATCCTGGACGGCGTTCACTACCTGCACT
CTAAGCGCATCGCACACTTTGACCTGAAGCCGGAAAACATCATGCTGCTG
GACAAGAACGTGCCCAACCCACGAATCAAGCTCATCGACTTCGGCATCGC
GCACAAGATCGAGGCGGGGAACGAGTTCAAGAACATCTTCGGCACCCCGG
AGTTTGTGGCCCCAGAGATTGTGAACTATGAGCCGCTGGGCCTGGAGGCG
GACATGTGGAGCATCGGTGTCATCACCTATATCCTCCTGAGCGGTGCATC
CCCGTTCCTGGGCGAGACCAAGCAGGAGACGCTCACCAACATCTCAGCCG
TGAACTACGACTTCGACGAGGAGTACTTCAGCAACACCAGCGAGCTGGCC
AAGGACTTCATTCGCCGGCTGCTCGTCAAAGATCCCAAGCGGAGAATGAC
CATTGCCCAGAGCCTGGAACATTCCTGGATTAAGGCGATCCGGCGGCGGA
ACGTGCGTGGTGAGGACAGCGGCCGCAAGCCCGAGCGGCGGCGCCTGAAG
ACCACGCGTCTGAAGGAGTACACCATCAAGTCGCACTCCAGCTTGCCGCC
CAACAACAGCTACGCCGACTTCGAGCGCTTCTCCAAGGTGCTGGAGGAGG
CGGCGGCCGCCGAGGAGGGCCTGCGCGAGCTGCAGCGCAGCCGGCGGCTC
TGCCACGAGGACGTGGAGGCGCTGGCCGCCATCTACGAGGAGAAGGAGGC
CTGGTACCGCGAGGAGAGCGACAGCCTGGGCCAGGACCTGCGGAGGCTAC
GGCAGGAGCTGCTCAAGACCGAGGCGCTCAAGCGGCAGGCGCAGGAGGAG
GCCAAGGGCGCGCTGCTGGGGACCAGCGGCCTCAAGCGCCGCTTCAGCCG
CCTGGAGAACCGCTACGAGGCGCTGGCCAAGCAAGTAGCCTCCGAGATGC
GCTTCGTGCAGGACCTCGTGCGCGCCCTGGAGCAGGAGAAGCTGCAGGGC
GTGGAGTGCGGGCTGCGCTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTFRQEDVEDHYEMGEEL
GSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILR
EIRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQF
LKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIE
AGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLG
ETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQS
LEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSY
ADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYEEKEAWYRE
ESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENR
YEALAKQVASEMRFVQDLVRALEQEKLQGVECGLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 79357 Daltons