cDNA Clone BRCA1

• Plasmid: pGEX6P-3-BRCA1
• Parent Plasmid: pGEX6P3
• Expressed: GST BRCA1
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACGGATCCATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAA TGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGT TGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTT TGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTT ATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTA GTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGAC ACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAA CTCTCCTGAACATCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCT ACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCC TTGCAGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAG AACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACA TTGAATTGGGATCTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTAT TGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAG GGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTG AGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTG AACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGG TAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATG CCAGCTCATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGA ATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTT AGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATG ATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCC CTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAA TCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTC AGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGAT GACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATT GGACGTTCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAG ACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGA GTTCACTCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAA AACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAA ATCTAATTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGT CCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCA TCCTGAGGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTG AAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATG AATATTACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCA GAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTT TCAAAACGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTC GAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAG GAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAA ATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGC AGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAG CAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGA AGAGTAACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGTGATACT TTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTC AAATACCAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAG AAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGAC CCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATC TGTAGAGAGTAGCAGTATTTCACTGGTACCTGGTACTGATTATGGCACTC AGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACA GAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGG ACTAATTCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTA AGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAA ATGGAAGAAAGTGAACTCGATGCTCAGTATTTGCAGAATACATTCAAGGT TTCAAAGCGCCAGTCATTTGCTCTGTTTTCAAATCCAGGAAATGCAGAAG AGGAATGTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGT CCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAA TGAGTCTAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTC CTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATC AAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAAC TGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTA TACCACCACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAA AATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGA AATGGGAAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATA ACATTAGAGAAAATGTTTTTAAAGGAGCCAGCTCAAGCAATATTAATGAA GTAGGTTCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTC CAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAAT TGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAA AGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATA TGAAGAAGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTT CAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGT TCTGAGACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATAC TAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAA GCGTCCAGAGAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACA CATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGA AGAGAACTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGT TATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACC GTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATT GAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCAT CTCAGGAACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTT TCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCA GGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAA GCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAA AGAGGAACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTC AAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTG AAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAG AGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGA ACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACC CTTCCATCATAAGTGACTCCTCTGCCCTTGAGGACCTGCGAAATCCAGAA CAAAGCACATCAGAAAAAGATTCGCATATACATGGCCAAAGGGACAACTC CATGTTTTCTAAAAGGCCTAGAGAACATATATCAGTATTAACTTCACAGA AAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGAC AAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGG AGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGT ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA GAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTC TGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAG AGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCT GAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAA CATACCATCTTCAACCTCTGCATTGAAATTTCCCCAATTGAAAGTTGCAG AATCTGCCCAGGGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTAT AATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTC AACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCC CAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACT TTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGA TGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGG GAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAA AGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAA TGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAA AGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATG CCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGT GAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGG TTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGG CAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGT AGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCC CCCACAGCCACTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDGSMDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQC
PLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGT
VRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKY
QGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCS
ENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIF
GKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDS
IQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVR
HSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTE
RSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTF
KVSKRQSFALFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGN
ETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKGASSSNINEVGSSTNEVGSSINEI
GSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKE
DTSFAENDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLL
SLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSM
DSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKDSHIHGQRD
NSMFSKRPREHISVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLE
ESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKFPQLKVAESAQGPAAAHTTDTAGYNAMEESVSREKPELT
ASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDV
VNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLD
SVALYQCQELDTYLIPQIPHSHY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32217
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No