cDNA Clone UBA7

• Plasmid: pET156P UBA7
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBA7
• Genbank: NP_003326.2
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCGCGATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTG
TATTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAGAGGATTCA
GGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGG
CCAAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCC
CACCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCA
GGACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGC
TCAACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGAC
CTGCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGA
GCAGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGG
CGGCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAG
GACTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCAT
CCAGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGG
CCAATACCCACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATT
GAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCG
GGAGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACT
TGCGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACAT
AAGTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTC
CCAGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGC
ACAAGTTCCAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTT
GATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCACTGAAGCG
GACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGA
CAGTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTG
GGTGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCAT
GCCTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAG
ATGGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGC
TATGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAGAAACTGAG
ACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGC
TCAAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTG
ACTGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTT
CCTCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAG
CAGCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTAC
CCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCG
TGTGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATG
TGGCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACA
TCGGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGC
CTACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTG
TCTGTACCGTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGG
GCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATCAA
CCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGA
CACTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAG
AACTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTT
TCATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGC
TTGAGGATGGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCC
TTGGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTACTGGCAGC
TGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGA
CTGCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAG
ATGGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGA
GTTTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGA
GTGTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGC
AACTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAA
CTACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCC
AGATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTG
GGCCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTT
TCGCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGC
CTTTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGG
GACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCT
GCTGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGC
ACGGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCC
CAGCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCA
GGCACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGG
GTGACGACGAGGACACTGCCTTCCCACCTCTGCACTATGAGCTGTAGGCG
GCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMDALDASKLLDEELY
SRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPH
PTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDL
LLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGED
FTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIE
GMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHK
SLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVD
AETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLG
AVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRY
DGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLT
VVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYP
LDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTS
GTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWA
RHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQN
WQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPL
EFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQM
APIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSN
FHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLG
LELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWD
RLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQ
HLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed protein : 115236 Da Daltons