cDNA Clone GFAT2

• Plasmid: pEBG6P-GFAT2
• Parent Plasmid: pEBG6P
• Expressed: GST GFAT2
• Genbank: NP_005101.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTGCGGAATCTTTGCCTACATGAACTACAGAGTCCCCCG
GACGAGGAAGGAGATCTTCGAAACCCTCATCAAGGGCCTGCAGCGGCTGG
AGTACAGAGGCTACGACTCGGCAGGTGTGGCGATCGATGGGAATAATCAC
GAAGTCAAAGAAAGACACATTCAGCTGGTCAAGAAAAGGGGGAAAGTCAA
GGCTCTCGATGAAGAACTTTACAAACAAGACAGCATGGACTTAAAAGTGG
AGTTTGAGACACACTTCGGCATTGCCCACACGCGCTGGGCCACCCACGGG
GTCCCCAGTGCTGTCAACAGCCACCCTCAGCGCTCAGACAAAGGCAACGA
ATTTGTTGTCATCCACAATGGGATCATCACAAATTACAAAGATCTGAGGA
AATTTCTGGAAAGCAAAGGCTACGAGTTTGAGTCAGAAACAGATACAGAG
ACCATCGCCAAGCTGATTAAATATGTGTTCGACAACAGAGAAACTGAGGA
CATTACGTTTTCAACGTTGGTCGAGAGAGTCATTCAGCAGTTGGAAGGTG
CATTCGCGCTGGTTTTCAAGAGTGTCCACTACCCAGGAGAAGCCGTTGCC
ACACGGAGAGGCAGCCCCCTGCTCATCGGAGTCCGGAGCAAATACAAGCT
CTCCACAGAACAGATCCCTATCTTATACAGGACGTGCACTCTGGAGAATG
TGAAGAATATCTGTAAGACACGGATGAAGAGGCTGGACAGCTCCGCCTGC
CTGCATGCTGTGGGCGACAAGGCCGTGGAATTCTTCTTTGCTTCTGATGC
AAGCGCTATCATAGAGCACACCAACCGGGTCATCTTCCTGGAGGACGATG
ACATCGCCGCAGTGGCTGATGGGAAACTCTCCATTCACCGGGTCAAGCGC
TCGGCCAGTGATGACCCATCTCGAGCCATCCAGACCTTGCAGATGGAACT
GCAGCAAATCATGAAAGGTAACTTCAGTGCGTTTATGCAGAAGGAGATCT
TCGAACAGCCAGAATCAGTTTTCAATACTATGAGAGGTCGGGTGAATTTT
GAAACCAACACAGTGCTCCTGGGTGGCTTGAAGGACCACTTGAAGGAGAT
TCGACGATGCCGACGGCTCATCGTGATTGGCTGTGGAACCAGCTACCACG
CTGCCGTGGCTACGCGGCAAGTTTTGGAGGAACTGACTGAGCTTCCTGTG
ATGGTTGAACTTGCTAGTGATTTTCTGGACAGGAACACACCTGTGTTCAG
GGATGACGTTTGCTTTTTCATCAGCCAGTCAGGCGAGACCGCGGACACCC
TCCTGGCGCTGCGCTACTGTAAGGACCGCGGCGCTCTCACCGTGGGCGTC
ACCAACACCGTGGGCAGCTCCATCTCTCGCGAGACCGACTGCGGCGTCCA
CATCAACGCAGGGCCGGAGATCGGCGTGGCCAGCACCAAGGCTTATACCA
GTCAGTTCATCTCTCTGGTGATGTTTGGTTTGATGATGTCTGAAGACCGA
ATTTCACTACAAAACAGGAGGCAAGAGATCATCCGTGGCTTGAGATCTTT
ACCTGAGCTGATCAAGGAAGTGCTGTCTCTGGAGGAGAAGATCCACGACT
TGGCCCTGGAGCTCTACACGCAGAGATCGCTGCTGGTGATGGGGCGGGGC
TACAACTATGCCACCTGCCTGGAAGGAGCCCTGAAAATTAAAGAGATAAC
CTACATGCACTCAGAAGGCATCCTGGCTGGGGAGCTGAAGCACGGGCCCC
TGGCACTGATTGACAAGCAGATGCCCGTCATCATGGTCATTATGAAGGAT
CCTTGCTTCGCCAAATGCCAGAACGCCCTGCAGCAAGTCACGGCCCGCCA
GGGTCGCCCCATTATACTGTGCTCCAAGGACGATACTGAAAGTTCCAAGT
TTGCGTATAAGACAATTGAGCTGCCCCACACTGTGGACTGCCTCCAGGGC
ATCCTGAGCGTGATTCCGCTGCAGCTGCTGTCCTTCCACCTGGCTGTTCT
CCGAGGATATGACGTTGACTTCCCCAGAAATCTGGCCAAGTCTGTAACTG
TGGAATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMCGI
FAYMNYRVPRTRKEIFETLIKGLQRLEYRGYDSAGVAIDGNNHEVKERHI
QLVKKRGKVKALDEELYKQDSMDLKVEFETHFGIAHTRWATHGVPSAVNS
HPQRSDKGNEFVVIHNGIITNYKDLRKFLESKGYEFESETDTETIAKLIK
YVFDNRETEDITFSTLVERVIQQLEGAFALVFKSVHYPGEAVATRRGSPL
LIGVRSKYKLSTEQIPILYRTCTLENVKNICKTRMKRLDSSACLHAVGDK
AVEFFFASDASAIIEHTNRVIFLEDDDIAAVADGKLSIHRVKRSASDDPS
RAIQTLQMELQQIMKGNFSAFMQKEIFEQPESVFNTMRGRVNFETNTVLL
GGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASD
FLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVTNTVGSS
ISRETDCGVHINAGPEIGVASTKAYTSQFISLVMFGLMMSEDRISLQNRR
QEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCL
EGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQ
NALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPL
QLLSFHLAVLRGYDVDFPRNLAKSVTVE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32078
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 105133 Daltons