cDNA Clone MKK7

• Plasmid: pEBG6P-MKK7
• Parent Plasmid: pEBG6P
• Expressed: GST MKK7
• Genbank: NP_005034
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGTCCTCCCTGGAACAGAAGCTGTCCCGCCTGGAAGC
AAAGCTGAAGCAGGAGAACCGGGAGGCCCGGCGGAGGATCGACCTCAACC
TGGATATCAGCCCCCAGCGGCCCAGGCCCACCCTGCAGCTCCCGCTGGCC
AACGATGGGGGCAGCCGCTCGCCATCCTCAGAGAGCTCCCCGCAGCACCC
CACGCCCCCCGCCCGGCCCCGCCACATGCTGGGGCTCCCGTCAACCCTGT
TCACACCCCGCAGCATGGAGAGCATTGAGATTGACCAGAAGCTGCAGGAG
ATCATGAAGCAGACGGGCTACCTGACCATCGGGGGCCAGCGCTACCAGGC
AGAAATCAACGACCTGGAGAACTTGGGCGAGATGGGCAGCGGCACCTGCG
GCCAGGTGTGGAAGATGCGCTTCCGGAAGACCGGCCACGTCATTGCCGTT
AAGCAAATGCGGCGCTCCGGGAACAAGGAGGAGAACAAGCGCATCCTCAT
GGACCTGGATGTGGTGCTGAAGAGCCACGACTGCCCCTACATCGTGCAGT
GCTTTGGGACGTTCATCACCAACACGGACGTCTTCATCGCCATGGAGCTC
ATGGGCACCTGCGCTGAGAAGCTCAAGAAGCGGATGCAGGGCCCCATCCC
CGAGCGCATTCTGGGCAAGATGACAGTGGCGATTGTGAAGGCGCTGTACT
ACCTGAAGGAGAAGCACGGTGTCATCCACCGCGACGTCAAGCCCTCCAAC
ATCCTGCTGGACGAGCGGGGCCAGATCAAGCTCTGCGACTTCGGCATCAG
CGGCCGCCTGGTGGACTCCAAAGCCAAGACGCGGAGCGCCGGCTGTGCCG
CCTACATGGCACCCGAGCGCATTGACCCCCCAGACCCCACCAAGCCGGAC
TATGACATCCGGGCCGACGTATGGAGCCTGGGCATCTCGTTGGTGGAGCT
GGCAACAGGACAGTTTCCCTACAAGAACTGCAAGACGGACTTTGAGGTCC
TCACCAAAGTCCTACAGGAAGAGCCCCCGCTTCTGCCCGGACACATGGGC
TTCTCGGGGGACTTCCAGTCCTTCGTCAAAGACTGCCTTACTAAAGATCA
CAGGAAGAGACCAAAGTATAATAAGCTACTTGAACACAGCTTCATCAAGC
GCTACGAGACGCTGGAGGTGGACGTGGCGTCCTGGTTCAAGGATGTCATG
GCGAAGACTGAGTCACCGCGGACTAGCGGCGTCCTGAGCCAGCCCCACCT
GCCCTTCTTCAGGTAGGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAASSLEQKLSRL
EAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQ
HPTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRY
QAEINDLENLGEMGSGTCGQVWKMRFRKTGHVIAVKQMRRSGNKEENKRI
LMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGP
IPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFG
ISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLV
ELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTK
DHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQP
HLPFFR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15964
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 74901 Daltons