cDNA Clone CRTC2

• Synonyms: TORC2
• Plasmid: pEGFP-C1- FLAG CRTC2 S274A
• Parent Plasmid: pEGFPC1
• Expressed: GFP FLAG CRTC2 S274A
• Tag: GFP
• Tag: FLAG

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGatggcgacgtc
gggggcgaacgggcctggttcggccacggcctcggcttccaatccgcgca
aatttagtgagaagattgcgctgcagaagcagcgtcaggccgaggagacg
gcggccttcgaggaggtgatgatggacatcggctccacccggttacaggc
ccaaaaactgcgactggcatacacaaggagctctcattatggtgggtctc
tgcccaatgttaaccagattggctctggcctggccgagttccagagcccc
ctccactcacctttggattcatctcggagcactcggcaccatgggctggt
ggaacgggtgcagcgagatcctcgaagaatggtgtccccacttcgccgat
acacccgccacattgacagctctccctatagtcctgcctacttatctcct
cccccagagtctagctggcgaaggacgatggcctggggcaatttccctgc
agagaaggggcagttgtttcgactaccatctgcacttaacaggacaagct
ctgactctgcccttcatacaagtgtgatgaaccccagtccccaggatacc
tacccaggccccacacctcccagcatcctgcccagccgacgtgggggtat
tctggatggtgaaatggaccccaaagtacctgctattgaggagaacttgc
tagatgacaagcatttgctgaagccatgggatgctaagaagctatcctca
tcctcttcccgacctcggtcctgtgaagtccctggaattaacatctttcc
atctcctgaccagcctgccaatgtgcctgtcctcccacctgccatgaaca
cggggggcgccctacctgacctcaccaacctgcactttcccccaccactg
cccacccccctggaccctgaagagacagcctaccctagcctgagtggggg
caacagtacctccaatttgacccacaccatgactcacctgggcatcagca
ggggcatgggcctgggcccaggctatgatgcaccaggacttcattcacct
ctcagccacccatccctgcagtcctccctaagcaatcccaacctccaggc
ttccctgagcagtcctcagccccagcttcagggctcccacagccacccct
ctctgcctgcctcctccttggcccgccatgtactgcccaccacctccctg
ggccacccctcactcagtgctccggctctttcctcctcctcttcctcctc
ctccacttcatctcctgttttgggcgccccctcttaccctgcttctaccc
ctggggcctccccccaccaccgccgtgtgcccctcagccccctgagtttg
ctcgcgggcccagccgacgccagaaggtcccaacagcagctgcccaaaca
gttttcgccaacaatgtcacccaccttgtcttccatcactcaaggcgtcc
ccctggataccagtaaactgtccactgaccagcggttacccccataccca
tacagctccccaagtctggttctgcctacccagccccacaccccaaagtc
tctacagcagccagggctgccctctcagtcttgttcagtgcagtcctcag
gtgggcagcccccaggcaggcagtctcattatgggacaccgtacccacct
gggcccagtgggcatgggcaacagtcttaccaccggccaatgagtgactt
caacctggggaatctggagcagttcagcatggagagcccatcagccagcc
tggtgctggatccccctggcttttctgaagggcctggatttttagggggt
gaggggccaatgggtggcccccaggatccccacaccttcaaccaccagaa
cttgacccactgttcccgccatggctcagggcctaacatcatcctcacag
gggactcctctccaggtttctctaaggagattgcagcagccctggccgga
gtgcctggctttgaggtgtcagcagctggattggagctagggcttgggct
agaagatgagctgcgcatggagccactgggcctggaagggctaaacatgc
tgagtgacccctgtgccctgctgcctgatcctgctgtggaggagtcattc
cgcagtgaccggctccaatgaGCGGCCGCGCAAGGGCGAATTCTGCAGTC
GACGGTACCGCGGGCCCGGGATCCACCGGATCTAGATAACTGATCATAAT
CAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCAC
ACCTCCCCCTGAACAGAAACAATATAAGAATTATAGGCAAGGGCGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFEFATMDYKDDDDKMATSGANGPGSATASASNPRKFSEKIALQKQRQAEET
AAFEEVMMDIGSTRLQAQKLRLAYTRSSHYGGSLPNVNQIGSGLAEFQSPLHSPLDSSRSTRHHGLVERVQRDPRRMVSPLRRYTRHIDSSPYSPAYLSP
PPESSWRRTMAWGNFPAEKGQLFRLPSALNRTSSDSALHTSVMNPSPQDTYPGPTPPSILPSRRGGILDGEMDPKVPAIEENLLDDKHLLKPWDAKKLSS
SSSRPRSCEVPGINIFPSPDQPANVPVLPPAMNTGGALPDLTNLHFPPPLPTPLDPEETAYPSLSGGNSTSNLTHTMTHLGISRGMGLGPGYDAPGLHSP
LSHPSLQSSLSNPNLQASLSSPQPQLQGSHSHPSLPASSLARHVLPTTSLGHPSLSAPALSSSSSSSSTSSPVLGAPSYPASTPGASPHHRRVPLSPLSL
LAGPADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLSTDQRLPPYPYSSPSLVLPTQPHTPKSLQQPGLPSQSCSVQSSGGQPPGRQSHYGTPYPP
GPSGHGQQSYHRPMSDFNLGNLEQFSMESPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQNLTHCSRHGSGPNIILTGDSSPGFSKEIAAALAG
VPGFEVSAAGLELGLGLEDELRMEPLGLEGLNMLSDPCALLPDPAVEESFRSDRLQ*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU15747
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee