cDNA Clone USP16

• Plasmid: pFBDual6P-1 USP16 Q141H E479D
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST USP16 Q141H E479D
• Genbank: AAH30777.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGA
TTCCTCTGAAACTTTAGAACCTGTGTGCAGACACATTAGAAAAGGATTGG
AACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATATCTGC
CAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAAC
AGAAGAAAAGCCTTCAGTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCT
GTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGCACTATCTGACGCCA
AGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATG
GTGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGG
GTCAAGTGGTTGATTATGTCAGAAAACATGCCAGCATTACAACTCCAAAG
CCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAGAAAA
AGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAG
AGAATCCTCCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGT
AATTTGGGAAACACATGTTTCTTCAATGCAGTTATGCAGAACTTGTCACA
AACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAA
TTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATA
AACCTTGAGCCTCCAGGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAA
TGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTCTTTT
CTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGAC
AGCCAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACA
CCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGGTAATTCTACTGAAA
AGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAA
TCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTAT
GATCATGTGTGATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCC
TTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAGTGTA
AATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGA
GGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTT
CTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGAAACAAGCCAAAAAG
CAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCA
TTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAG
CTGAAATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCA
CAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATTTGAA
TGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAG
AGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTA
ACATCTTCTCCCACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAG
CAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATG
CTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGC
TTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGTTCAA
TCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCG
AATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAA
GGCAAATATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGC
AGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGA
TTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTT
TCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATG
TTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAA
CACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAG
AACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCAC
AAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACA
CATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCT
CCTATTTTATGAGAGAATACTGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGKKRTKGKTVPIDDSSET
LEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKP
SVWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVC
DNEVQYCSSNQLGQVVDYVRKHASITTPKPEKDNGNIELENKKLEKESKN
EQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVL
RELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQE
TKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVS
KGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCD
QCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKD
NDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDI
CTIDHPEDSDNEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAK
MIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEV
DISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTL
ANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIK
GERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEIL
DLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANS
HLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYE
RIL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15655
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression.; ; Estimated MW of expressed protein : 120266 Daltons