cDNA Clone USP16

• Plasmid: pFBHTb USP16 Q141H E479D
• Parent Plasmid: pFastBac HTb
• Expressed: 6His USP16 Q141H E479D
• Genbank: AAH30777.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGA
TTCCTCTGAAACTTTAGAACCTGTGTGCAGACACATTAGAAAAGGATTGG
AACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATATCTGC
CAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAAC
AGAAGAAAAGCCTTCAGTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCT
GTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGCACTATCTGACGCCA
AGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATG
GTGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGG
GTCAAGTGGTTGATTATGTCAGAAAACATGCCAGCATTACAACTCCAAAG
CCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAGAAAA
AGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAG
AGAATCCTCCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGT
AATTTGGGAAACACATGTTTCTTCAATGCAGTTATGCAGAACTTGTCACA
AACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAA
TTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATA
AACCTTGAGCCTCCAGGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAA
TGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTCTTTT
CTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGAC
AGCCAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACA
CCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGGTAATTCTACTGAAA
AGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAA
TCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTAT
GATCATGTGTGATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCC
TTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAGTGTA
AATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGA
GGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTT
CTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGAAACAAGCCAAAAAG
CAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCA
TTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAG
CTGAAATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCA
CAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATTTGAA
TGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAG
AGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTA
ACATCTTCTCCCACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAG
CAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATG
CTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGC
TTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGTTCAA
TCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCG
AATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAA
GGCAAATATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGC
AGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGA
TTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTT
TCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATG
TTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAA
CACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAG
AACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCAC
AAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACA
CATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCT
CCTATTTTATGAGAGAATACTGTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMGKKRTKGKTVPIDDSSETLEP
VCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPSVW
LCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNE
VQYCSSNQLGQVVDYVRKHASITTPKPEKDNGNIELENKKLEKESKNEQE
REKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLREL
LKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKK
GVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGI
LKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCR
TVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDS
YIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTI
DHPEDSDNEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIE
SVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDIS
NGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANR
EVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGER
KHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLA
PFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLS
NLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL
*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15654
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 96814 Daltons