cDNA Clone NEK6

• Plasmid: pEBG6P-NEK6
• Parent Plasmid: pEBG6P
• Expressed: GST-NEK6
• Genbank: NM_014397
• Tag: GST
• Species: Human

Sequence of Insert

ATGCCCCATGGAGGGAGTTCCAACAACCTCTGCCACACCCTGGGGCCTGT
GCATCCTCCTGACCCACAGAGGCATCCCAACACGCTGTCTTTTCGCTGCT
CGCTGGCGGACTTCCAGATCGAAAAGAAGATAGGCCGAGGACAGTTCAGC
GAGGTGTACAAGGCCACCTGCCTGCTGGACAGGAAGACAGTGGCTCTGAA
GAAGGTGCAGATCTTTGAGATGATGGACGCCAAGGCGAGGCAGGACTGTG
TCAAGGAGATCGGCCTCTTGAAGCAACTGAACCACCCAAATATCATCAAG
TATTTGGACTCGTTTATCGAAGACAACGAGCTGAACATTGTGCTGGAGTT
GGCTGACGCAGGGGACCTCTCGCAGATGATCAAGTACTTTAAGAAGCAGA
AGCGGCTCATCCCGGAGAGGACAGTATGGAAGTACTTTGTGCAGCTGTGC
AGCGCCGTGGAGCACATGCATTCACGCCGGGTGATGCACCGAGACATCAA
GCCTGCCAACGTGTTCATCACAGCCACGGGCGTCGTGAAGCTCGGTGACC
TTGGTCTGGGCCGCTTCTTCAGCTCTGAGACCACCGCAGCCCACTCCCTA
GTGGGGACGCCCTACTACATGTCACCGGAGAGGATCCATGAGAACGGCTA
CAACTTCAAGTCCGACATCTGGTCCTTGGGCTGTCTGCTGTACGAGATGG
CAGCCCTCCAGAGCCCCTTCTATGGAGATAAGATGAATCTCTTCTCCCTG
TGCCAGAAGATCGAGCAGTGTGACTACCCCCCACTCCCCGGGGAGCACTA
CTCCGAGAAGTTACGAGAACTGGTCAGCATGTGCATCTGCCCTGACCCCC
ACCAGAGACCTGACATCGGATACGTGCACCAGGTGGCCAAGCAGATGCAC
ATCTGGATGTCCAGCACCTGA

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMPHG
GSSNNLCHTLGPVHPPDPQRHPNTLSFRCSLADFQIEKKIGRGQFSEVYK
ATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDS
FIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAVE
HMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSETTAAHSLVGTP
YYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKI
EQCDYPPLPGEHYSEKLRELVSMCICPDPHQRPDIGYVHQVAKQMHIWMS
ST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15289
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; ; Estimated MW of expressed protein : 63239 Daltons